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Calcium Imaging and Amperometric Recording in Cultured Chromaffin Cells and Adrenal Slices from Normotensive, Wistar Kyoto Rats and Spontaneously Hypertensive Rats

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Chromaffin Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2565))

Abstract

The spontaneously hypertensive rat (SHR) is a model widely used to investigate the causal mechanisms of essential hypertension. The enhanced catecholamine (CA) release reported in adrenal glands from adult SHRs raised considerable interest for its possible implication in the genesis of hypertension. The use of powerful techniques such as calcium imaging, electrophysiology, and single-cell amperometry to monitor in real time the key steps in CA secretion has allowed a better understanding of the role of chromaffin cells (CC) in the pathophysiology of hypertension, although several questions remain. Additionally, the implementation of these techniques in preparations in situ, such as the acute adrenal gland slice, which maintains the microenvironment, cell-to-cell communication, and anatomical structure similar to that of the intact adrenal gland, yields data that may have even greater physiological relevance. Here, we describe the procedures to measure the blood pressure of rats in a noninvasive manner, how to obtain primary cultures of adrenal chromaffin cells and acute adrenal slices, and how to perform amperometric recordings and intracellular calcium imaging in these preparations.

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Author information

Authors and Affiliations

  1. Department of Biological Sciences, Columbia University, New York, NY, USA

    Tzitzitlini Alejandre-García

  2. Cátedras CONACyT, Laboratorio de Fisiología Celular, Instituto Nacional de Psiquiatría Ramón de La Fuente Muniz, México City, Mexico

    Pedro Segura-Chama

  3. Departamento Neurociencia Cognitiva, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México City, Mexico

    Oscar Javier Parada-Parra, Diana Millán-Aldaco & Arturo Hernández-Cruz

Authors
  1. Tzitzitlini Alejandre-García
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  2. Pedro Segura-Chama
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  3. Oscar Javier Parada-Parra
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  4. Diana Millán-Aldaco
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  5. Arturo Hernández-Cruz
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Corresponding author

Correspondence to Arturo Hernández-Cruz .

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Editors and Affiliations

  1. Department of Physical Medicine and Pharmacology, Universidad de La Laguna, La Laguna, Spain

    Ricardo Borges

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Alejandre-García, T., Segura-Chama, P., Parada-Parra, O.J., Millán-Aldaco, D., Hernández-Cruz, A. (2023). Calcium Imaging and Amperometric Recording in Cultured Chromaffin Cells and Adrenal Slices from Normotensive, Wistar Kyoto Rats and Spontaneously Hypertensive Rats. In: Borges, R. (eds) Chromaffin Cells. Methods in Molecular Biology, vol 2565. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2671-9_10

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  • DOI: https://doi.org/10.1007/978-1-0716-2671-9_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2670-2

  • Online ISBN: 978-1-0716-2671-9

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