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Processing and Analysis of Ascites

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Ovarian Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2424))

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Abstract

The accumulation of peritoneal fluid, referred to as ascites, is common in ovarian cancer. This fluid is a complex mixture that may include cells as well as a diverse array of cytokines and growth factors. Here we describe a comprehensive method to process ascites to maximize data collection. The cellular fraction and fluid are first separated by centrifugation. The fluid can be frozen for later analysis of soluble factors or for use in in vitro experiments. The cellular fraction can be processed to analyze its composition or stored for future use.

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Authors and Affiliations

  1. Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA

    Hannah M. Micek, Molly J. Carroll & Pamela K. Kreeger

  2. Department of Obstetrics and Gynecology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA

    Lisa Barroilhet

  3. University of Wisconsin Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA

    Lisa Barroilhet

  4. Department of Cell and Regenerative Biology, Department of Obstetrics and Gynecology, and University of Wisconsin Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA

    Pamela K. Kreeger

Authors
  1. Hannah M. Micek
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  2. Molly J. Carroll
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  3. Lisa Barroilhet
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  4. Pamela K. Kreeger
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Corresponding author

Correspondence to Pamela K. Kreeger .

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA

    Pamela K. Kreeger

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Micek, H.M., Carroll, M.J., Barroilhet, L., Kreeger, P.K. (2022). Processing and Analysis of Ascites. In: Kreeger, P.K. (eds) Ovarian Cancer. Methods in Molecular Biology, vol 2424. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1956-8_5

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  • DOI: https://doi.org/10.1007/978-1-0716-1956-8_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1955-1

  • Online ISBN: 978-1-0716-1956-8

  • eBook Packages: Springer Protocols

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