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Implementation of Ion Mobility Spectrometry-Based Separations in Structures for Lossless Ion Manipulations (SLIM)

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Biomedical Engineering Technologies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2394))

Abstract

Structures for Lossless Ion Manipulations (SLIM) is a powerful variant of traveling wave ion mobility spectrometry (TW-IMS) that uses a serpentine pattern of microelectrodes deposited onto printed circuit boards to achieve ultralong ion path lengths (13.5 m). Ions are propelled through SLIM platforms via arrays of TW electrodes while RF and DC electrodes provide radial confinement, establishing near lossless transmission. The recent ability to cycle ions multiple times through a SLIM has allowed ion path lengths to exceed 1000 m, providing unprecedented separation power and the ability to observe ion structural conformations unobtainable with other IMS technologies. The combination of high separation power, high signal intensity, and the ability to couple with mass spectrometry places SLIM in the unique position of being able to address longstanding proteomics and metabolomics challenges by allowing the characterization of isomeric mixtures containing low abundance analytes.

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Acknowledgments

We acknowledge the support from the National Cancer Institute (R33 CA217699). The work was performed at the W. R. Wiley Environmental Molecular Sciences Laboratory (EMSL), a DOE national scientific user facility at the Pacific Northwest National Laboratory (PNNL). PNNL is operated by Battelle under contract DE-AC05-76RL0 1830 for the DOE.

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  1. Adam Hollerbach and Christopher R. Conant contributed equally with all other contributors.

Authors and Affiliations

  1. Pacific Northwest National Laboratory, Richland, WA, USA

    Adam L. Hollerbach, Christopher R. Conant, Gabe Nagy & Yehia M. Ibrahim

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  1. Adam L. Hollerbach
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  2. Christopher R. Conant
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  3. Gabe Nagy
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  4. Yehia M. Ibrahim
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Corresponding author

Correspondence to Yehia M. Ibrahim .

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Editors and Affiliations

  1. Division of Cancer Treatment and Diagnosis, National Cancer Institute, Rockville, MD, USA

    Avraham Rasooly

  2. Division of Cancer Treatment and Diagnosis, National Cancer Institute, Rockville, MD, USA

    Houston Baker

  3. Division of Cancer Treatment and Diagnosis, National Cancer Institute, NIH, Bethesda, MD, USA

    Miguel R. Ossandon

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Hollerbach, A.L., Conant, C.R., Nagy, G., Ibrahim, Y.M. (2022). Implementation of Ion Mobility Spectrometry-Based Separations in Structures for Lossless Ion Manipulations (SLIM). In: Rasooly, A., Baker, H., Ossandon, M.R. (eds) Biomedical Engineering Technologies. Methods in Molecular Biology, vol 2394. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1811-0_23

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  • DOI: https://doi.org/10.1007/978-1-0716-1811-0_23

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