Abstract
Fusarium ranks as the most important group of plant pathogens, responsible for a wide range of economically destructive diseases, including vascular wilts and root, crown, and stem rots. In addition, head blight and ear rot diseases are associated with the accumulation of mycotoxins in cereals. With over 300 phylogenetically distinct species, and a dearth of phenotypical characteristics, DNA sequence data in most instances is the only reliable means for obtaining an accurate species identification. Here we describe how to obtain single-spored pure cultures from symptomatic host tissue and a molecular identification by querying publicly accessible DNA sequence databases using a portion of translation elongation factor 1-α (TEF1), the largest subunit of RNA polymerase (RPB1), and/or the second largest subunit of RNA polymerase (RPB2).
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Acknowledgments
The authors thank Amy McGovern and Crystal Probyn for skilled technical assistance. The US Department of Agriculture Agricultural Research Service (USDA-ARS) National Program for Food Safety and National Science Foundation (NSF; DEB-1655980) provided funding for this study. This research was supported in part by an appointment to the ARS Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the US Department of Energy (DOE) and the US Department of Agriculture (USDA). ORISE is managed by ORAU under DOE contract number DE-SC0014664. All opinions expressed in this paper are the authors’ and do not necessarily reflect the policies and views of USDA, DOE, or ORAU/ORISE.
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O’Donnell, K., Laraba, I., Geiser, D.M. (2022). Pure Culture and DNA Sequence-Based Identification of Fusarium from Symptomatic Plants and Diverse Substrates. In: Coleman, J. (eds) Fusarium wilt. Methods in Molecular Biology, vol 2391. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1795-3_1
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DOI: https://doi.org/10.1007/978-1-0716-1795-3_1
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