Abstract
Alzheimer’s disease (AD), Pick’s disease, fronto-temporal lobar degeneration, cortico-basal degeneration, and primary age related tauopathy are examples of neurodegenerative disorders with tau accumulation and jointly referred as “tauopathies.” The mechanisms through which tau leads to neurodegeneration are not fully understood but include conversion into toxic oligomers and protofibrils, cell-to-cell propagation, post-transcriptional modifications and as a mediator of cell death signals among others. Potential therapeutics includes reducing tau synthesis (e.g., anti-sense); targeting selective tau species and aggregates or blocking cell-to-cell transmission (e.g., antibodies) or by promoting clearance of tau (e.g., autophagy activators). Among them, immunotherapy is currently one of the approaches most actively explored including active, passive, and cellular. A potential problem with immunotherapy has been the trafficking of the antibodies into the CNS. In this chapter, we describe a method for the production and testing of viral vector driven, brain-penetrating, single chain antibodies that specifically recognize 3RTau. These single chain antibodies are modified by the addition of a fragment of the apoB protein to facilitate trafficking into the brain, once in the CNS these antibody fragments recognize tau with potential value for the treatment of AD and related dementias.
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References
Wang Y, Mandelkow E (2016) Tau in physiology and pathology. Nat Rev Neurosci 17:5–21
Ballatore C, Lee VM, Trojanowski JQ (2007) Tau-mediated neurodegeneration in Alzheimer’s disease and related disorders. Nat Rev Neurosci 8:663–672
Lee VM, Goedert M, Trojanowski JQ (2001) Neurodegenerative tauopathies. Annu Rev Neurosci 24:1121–1159
Gao Y, Tan L, Yu JT, Tan L (2017) Tau in Alzheimer’s disease: mechanisms and therapeutic strategies. Curr Alzheimer Res 15(3):283–300
Haass C, Selkoe DJ (2007) Soluble protein oligomers in neurodegeneration: lessons from the Alzheimer’s amyloid beta-peptide. Nat Rev Mol Cell Biol 8:101–112
De Strooper B, Karran E (2016) The cellular phase of Alzheimer’s disease. Cell 164:603–615
Sibener L, Zaganjor I, Snyder HM, Bain LJ, Egge R et al (2014) Alzheimer’s disease prevalence, costs, and prevention for military personnel and veterans. Alzheimers Dement 10:S105–S110
Alonso A d C, Iqbal K (2005) Tau-induced neurodegeneration: a clue to its mechanism. J Alzheimers Dis 8:223–226
Andreadis A, Brown WM, Kosik KS (1992) Structure and novel exons of the human tau gene. Biochemistry 31:10626–10633
Caillet-Boudin ML, Buee L, Sergeant N, Lefebvre B (2015) Regulation of human MAPT gene expression. Mol Neurodegener 10:28
Guo T, Noble W, Hanger DP (2017) Roles of tau protein in health and disease. Acta Neuropathol 133:665–704
Olney NT, Spina S, Miller BL (2017) Frontotemporal Dementia. Neurol Clin 35:339–374
Arendt T, Stieler JT, Holzer M (2016) Tau and tauopathies. Brain Res Bull 126:238–292
Tacik P, Sanchez-Contreras M, Rademakers R, Dickson DW, Wszolek ZK (2016) Genetic disorders with tau pathology: a review of the literature and report of two patients with Tauopathy and positive family histories. Neurodegener Dis 16:12–21
Dickson DW, Kouri N, Murray ME, Josephs KA (2011) Neuropathology of frontotemporal lobar degeneration-tau (FTLD-tau). J Mol Neurosci 45:384–389
Terry R, Hansen L, Masliah E (1994) Structural basis of the cognitive alterations in Alzheimer disease. In: Terry R, Katzman R (eds) Alzheimer disease. Raven Press, New York, pp 179–196
Overk CR, Masliah E (2014) Pathogenesis of synaptic degeneration in Alzheimer’s disease and Lewy body disease. Biochem Pharmacol 88:508–516
Selkoe DJ, Hardy J (2016) The amyloid hypothesis of Alzheimer’s disease at 25 years. EMBO Mol Med 8:595–608
Mucke L, Selkoe DJ (2012) Neurotoxicity of amyloid beta-protein: synaptic and network dysfunction. Cold Spring Harb Perspect Med 2:a006338
Kovacs GG (2015) Invited review: neuropathology of tauopathies: principles and practice. Neuropathol Appl Neurobiol 41:3–23
Takeda N, Kishimoto Y, Yokota O (2012) Pick’s disease. Adv Exp Med Biol 724:300–316
Ward SM, Himmelstein DS, Lancia JK, Binder LI (2012) Tau oligomers and tau toxicity in neurodegenerative disease. Biochem Soc Trans 40:667–671
Mohamed NV, Herrou T, Plouffe V, Piperno N, Leclerc N (2013) Spreading of tau pathology in Alzheimer’s disease by cell-to-cell transmission. Eur J Neurosci 37:1939–1948
Gibbons GS, Lee VMY, Trojanowski JQ (2019) Mechanisms of cell-to-cell transmission of pathological tau: a review. JAMA Neurol 76:101–108
Martin L, Latypova X, Terro F (2011) Post-translational modifications of tau protein: implications for Alzheimer’s disease. Neurochem Int 58:458–471
Morris M, Knudsen GM, Maeda S, Trinidad JC, Ioanoviciu A et al (2015) Tau post-translational modifications in wild-type and human amyloid precursor protein transgenic mice. Nat Neurosci 18:1183–1189
Feinstein SC, Wilson L (2005) Inability of tau to properly regulate neuronal microtubule dynamics: a loss-of-function mechanism by which tau might mediate neuronal cell death. Biochim Biophys Acta 1739:268–279
Pedersen JT, Sigurdsson EM (2015) Tau immunotherapy for Alzheimer’s disease. Trends Mol Med 21:394–402
Valera E, Spencer B, Masliah E (2016) Immunotherapeutic approaches targeting amyloid-beta, alpha-Synuclein, and tau for the treatment of neurodegenerative disorders. Neurotherapeutics 13:179–189
Boado RJ, Lu JZ, Hui EK, Sumbria RK, Pardridge WM (2013) Pharmacokinetics and brain uptake in the rhesus monkey of a fusion protein of arylsulfatase a and a monoclonal antibody against the human insulin receptor. Biotechnol Bioeng 110:1456–1465
Spencer B, Verma I, Desplats P, Morvinski D, Rockenstein E et al (2014) A neuroprotective brain-penetrating endopeptidase fusion protein ameliorates Alzheimer disease pathology and restores neurogenesis. J Biol Chem 289:17917–17931
Tian H, Davidowitz E, Lopez P, He P, Schulz P et al (2015) Isolation and characterization of antibody fragments selective for toxic oligomeric tau. Neurobiol Aging 36:1342–1355
Messer A, Butler DC (2020) Optimizing intracellular antibodies (intrabodies/nanobodies) to treat neurodegenerative disorders. Neurobiol Dis 134:104619
Spencer B, Bruschweiler S, Sealey-Cardona M, Rockenstein E, Adame A et al (2018) Selective targeting of 3 repeat tau with brain penetrating single chain antibodies for the treatment of neurodegenerative disorders. Acta Neuropathol 136:69–87
Hussain MM, Strickland DK, Bakillah A (1999) The mammalian low-density lipoprotein receptor family. Annu Rev Nutr 19:141–172
Bickel U, Yoshikawa T, Pardridge WM (2001) Delivery of peptides and proteins through the blood-brain barrier. Adv Drug Deliv Rev 46:247–279
Spencer B, Emadi S, Desplats P, Eleuteri S, Michael S et al (2014) ESCRT-mediated uptake and degradation of brain-targeted alpha-synuclein single chain antibody attenuates neuronal degeneration in vivo. Mol Ther 22:1753–1767
Spencer B, Marr RA, Gindi R, Potkar R, Michael S et al (2011) Peripheral delivery of a CNS targeted, metalo-protease reduces abeta toxicity in a mouse model of Alzheimer’s disease. PLoS One 6:e16575
Spencer B, Potkar R, Metcalf J, Thrin I, Adame A et al (2016) Systemic central nervous system (CNS)-targeted delivery of neuropeptide Y (NPY) reduces neurodegeneration and increases neural precursor cell proliferation in a mouse model of Alzheimer disease. J Biol Chem 291:1905–1920
Spencer BJ, Verma IM (2007) Targeted delivery of proteins across the blood-brain barrier. Proc Natl Acad Sci U S A 104:7594–7599
Rockenstein E, Overk CR, Ubhi K, Mante M, Patrick C et al (2015) A novel triple repeat mutant tau transgenic model that mimics aspects of Pick’s disease and Fronto-temporal Tauopathies. PLoS One 10:e0121570
Spencer B, Michael S, Shen J, Kosberg K, Rockenstein E et al (2013) Lentivirus mediated delivery of neurosin promotes clearance of wild-type alpha-synuclein and reduces the pathology in an alpha-synuclein model of LBD. Mol Ther 21:31–41
Jones NH, Clabby ML, Dialynas DP, Huang HJ, Herzenberg LA et al (1986) Isolation of complementary DNA clones encoding the human lymphocyte glycoprotein T1/Leu-1. Nature 323:346–349
Masliah E, Spencer B (2015) Applications of ApoB LDLR-binding domain approach for the development of CNS-penetrating peptides for Alzheimer’s disease. Methods Mol Biol 1324:331–337
Masliah E, Spencer B, Rockenstein E (2017) Compositions targeting 3-repeat tau for the treatment of neurodegenerative disorders, and methods for making and using them. WIPO, USA, New York
Spencer B, Desplats PA, Overk CR, Valera-Martin E, Rissman RA et al (2016) Reducing endogenous alpha-Synuclein mitigates the degeneration of selective neuronal populations in an Alzheimer’s disease transgenic mouse model. J Neurosci 36:7971–7984
Spencer B, Potkar R, Trejo M, Rockenstein E, Patrick C et al (2009) Beclin 1 gene transfer activates autophagy and ameliorates the neurodegenerative pathology in alpha-synuclein models of Parkinson’s and Lewy body diseases. J Neurosci 29:13578–13588
Masliah E, Rockenstein E, Veinbergs I, Mallory M, Hashimoto M et al (2000) Dopaminergic loss and inclusion body formation in alpha-synuclein mice: implications for neurodegenerative disorders. Science 287:1265–1269
Nisbet RM, Van der Jeugd A, Leinenga G, Evans HT, Janowicz PW et al (2017) Combined effects of scanning ultrasound and a tau-specific single chain antibody in a tau transgenic mouse model. Brain 140:1220–1230
Dupre E, Danis C, Arrial A, Hanoulle X, Homa M et al (2019) Single domain antibody fragments as new tools for the detection of neuronal tau protein in cells and in mice studies. ACS Chem Neurosci 10:3997–4006
Venkataraman L, He P, Schulz P, Sierks MR (2020) Isolation and characterization of antibody fragment selective for human Alzheimer’s disease brain-derived tau variants. Neurobiol Aging 94:7–14
Vitale F, Giliberto L, Ruiz S, Steslow K, Marambaud P et al (2018) Anti-tau conformational scFv MC1 antibody efficiently reduces pathological tau species in adult JNPL3 mice. Acta Neuropathol Commun 6:82
Krishnaswamy S, Huang HW, Marchal IS, Ryoo HD, Sigurdsson EM (2020) Neuronally expressed anti-tau scFv prevents tauopathy-induced phenotypes in drosophila models. Neurobiol Dis 137:104770
Spencer B, Valera E, Rockenstein E, Trejo-Morales M, Adame A et al (2015) A brain-targeted, modified neurosin (kallikrein-6) reduces alpha-synuclein accumulation in a mouse model of multiple system atrophy. Mol Neurodegener 10:48
Acknowledgments
Supported by NIH grants AG5131, AG018440, AG051839 to RR. HEK293 antibody production was performed by the VBCF Protein Technologies Facility (www.vbcf.ac.at).
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Spencer, B., Rissman, R.A., Overk, C., Masliah, E. (2022). Novel Brain-Penetrating Single Chain Antibodies Directed Against 3RTau for the Treatment of Alzheimer’s Disease and Related Dementias. In: Langel, Ü. (eds) Cell Penetrating Peptides. Methods in Molecular Biology, vol 2383. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1752-6_28
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DOI: https://doi.org/10.1007/978-1-0716-1752-6_28
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