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Phosphoproteomics Profiling of Receptor Kinase Mutants

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Plant Phosphoproteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2358))

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Abstract

The transmembrane receptor kinase family is the largest protein kinase family in Arabidopsis. Many members of this family play critical roles in plant signaling pathways. However, many of these kinases have yet uncharacterized functions and very little is known about the direct substrates of these kinases. We have developed the “ShortPhos” method, an efficient and simple mass spectrometry (MS)-based phosphoproteomics protocol to perform comparative phosphopeptide profiling of knockout mutants of receptor-like kinases. Through this method, we are able to better understand the functional roles of plant kinases in the context of their signaling networks.

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Author information

Authors and Affiliations

  1. State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, China

    Dandan Lu

  2. State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, China

    Leonard Krall

  3. Department of Plant Systems Biology, University of Hohenheim, Stuttgart, Germany

    Lin Xi

  4. State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, China

    Ting Gao & Xu Na Wu

Authors
  1. Dandan Lu
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  2. Ting Gao
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  3. Lin Xi
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  4. Leonard Krall
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  5. Xu Na Wu
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Corresponding author

Correspondence to Xu Na Wu .

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Editors and Affiliations

  1. State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, China

    Xu Na Wu

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Lu, D., Gao, T., Xi, L., Krall, L., Wu, X.N. (2021). Phosphoproteomics Profiling of Receptor Kinase Mutants. In: Wu, X.N. (eds) Plant Phosphoproteomics. Methods in Molecular Biology, vol 2358. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1625-3_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1625-3_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1624-6

  • Online ISBN: 978-1-0716-1625-3

  • eBook Packages: Springer Protocols

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