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Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods

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Neural Reprogramming

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2352))

Abstract

The detection of neurotransmitter release from reprogrammed human cell is an important demonstration of their functionality. Electrochemistry has the distinct advantages over alternative methods that it allows for the measuring of the analyte of interest at a high temporal resolution. This is necessary for fast events, such as neurotransmitter release and reuptake, which happen in the order of milliseconds to seconds. The precise description of these kinetic events can lead to insights into the function of cells in health and disease and allows for the exploration of events that might be missed using methods that look at absolute concentration values or methods that have a slower sampling rate. In the present chapter, we describe the use of constant potential amperometry and enzyme-coated multielectrode arrays for the detection of glutamate in vitro. These biosensors have the distinct advantage of “self-referencing,” a method providing high selectivity while retaining outstanding temporal resolution. Here, we provide a step-by-step user guide for a commercially available system and its application for in vitro systems such as reprogrammed cells.

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Acknowledgments

I would like to acknowledge my heavy reliance on excellent articles and reviews by the group of Greg Gerhardt (Kentucky University) and Erin Hascup (now Southern Illinois University) and especially the advice and support of Francois Pomerleau (Kentucky University) over the years. The Kentucky team is a great resource of expertise and an example on sharing and dissemination of knowledge. Furthermore, I would like to acknowledge Martin Lundblad for setting up the amperometry platform at Lund University as well as mentoring me from my start in Lund until the present day.

Financially, this work was supported by grants from the Swedish Research Council (VR), the Parkinsonsfonden, the Crafoord Stiftelsen, the Åke Wiberg Foundation, the Royal Physiographic Society in Lund, the Åhlensstiftelsen, the Jeanssons Foundation, and the Swedish Society for Medical Research (SSMF).

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  1. Behavioural Neuroscience Laboratory, Department of Experimental Medical Sciences, Lund University, Lund, Sweden

    Andreas Heuer

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  1. Andreas Heuer
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Correspondence to Andreas Heuer .

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  1. Stem Cells, Aging and Neurodegeneration Group, Faculty of Medicine, Department of Clinical Sciences, Neurology, Lund Stem Cell Center, Lund University, Lund, Sweden

    Henrik Ahlenius

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Heuer, A. (2021). Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods. In: Ahlenius, H. (eds) Neural Reprogramming. Methods in Molecular Biology, vol 2352. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1601-7_14

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  • DOI: https://doi.org/10.1007/978-1-0716-1601-7_14

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  • Publisher Name: Humana, New York, NY

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