Abstract
The emerging data indicates that long noncoding RNAs (lncRNAs) are involved in fundamental biological processes, and their deregulation may lead to oncogenesis and other diseases. LncRNA fulfil its biological functions at least in part by interacting with distinctive proteins. Here, we described two methods to identify the direct or indirect interactions between lncRNA and proteins: cross-linking and immunoprecipitation (CLIP) and RNA pull-down assay. CLIP methods enable yield a list of lncRNAs that directly interact target protein in living cells, whereas immunoprecipitation of biotin-labeled RNA (RNA pull-down) assay represents a method for identification of proteins that directly and indirectly bind with a particular target lncRNA of interest.
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Acknowledgments
This work was funded by the National Nature Science Foundation of China (No. 31301131, 31741084, and 31871389) and Basic Science Foundation of Science and Technology Innovation Commission in Shenzhen (No. JCYJ20170811154452255).
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Zhao, K., Wang, X., Hu, Y. (2021). Identification of lncRNA–Protein Interactions by CLIP and RNA Pull-Down Assays. In: Navarro, A. (eds) Long Non-Coding RNAs in Cancer. Methods in Molecular Biology, vol 2348. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1581-2_16
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DOI: https://doi.org/10.1007/978-1-0716-1581-2_16
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