Abstract
β-carotene is an increasingly sought-after organic pigment with antioxidant properties and a vitamin precursor. Yarrowia lipolytica, though unable to naturally synthesize carotenoids, can produce high amounts of the precursor acetyl-CoA making it a promising host for metabolic engineering towards novel biotechnological production of carotenoids. Here, we describe a synthetic biology methodology for Y. Lipolytica metabolic engineering based on Golden Gate DNA assembly for the generation of a multigene cassette, subsequent transformation enabling β-carotene biosynthesis, and quantification of the compound.
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Acknowledgments
R. Ledesma-Amaro received financial support from the Imperial College London in the form of an IC Research Fellowship.
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Pesantes-Munoz, M., Ledesma-Amaro, R. (2021). Pathway Engineering for Beta-Carotene and Carotenoid Biosynthesis in Y. lipolytica. In: Wheeldon, I., Blenner, M. (eds) Yarrowia lipolytica. Methods in Molecular Biology, vol 2307. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1414-3_13
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DOI: https://doi.org/10.1007/978-1-0716-1414-3_13
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