Abstract
The obstacles to breeding programs in Jatropha are the long reproductive cycle with a juvenile phase that lasts several months, the highly heterozygous nature of the genome, the large canopy size, and self-incompatibility that is a long-term process which requires multiple cycles of self-pollination to achieve complete homozygosity. In vitro plant tissue culture-based tools such as haploids and doubled haploid techniques can increase the selection efficiency, resulting into selection of superior plants with complete homozygosity in one generation. It bypasses the complications of greenhouse field evaluation or off-season generation advancement, which takes about 8–10 generations in traditional breeding with the time line of 10–12 years. The haploids have in fact a single set of chromosomes, which undergoes duplication spontaneously during in vitro culture conditions, and are further converted into doubled haploid plants. This represents a major biotechnological tool to accelerate plant breeding. Here, we have established a reproducible, unique anther culture protocol in Jatropha curcas to develop haploid and doubled haploid plants.
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Acknowledgments
The authors are grateful to the leadership of Dr. Santanu Dasgupta and Dr. Ajit Sapre for the support and encouragement at the Reliance Industries Ltd., India. S.A. wish to thank Dr. Saakshi Jalali for proof reading and evaluating this paper.
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Arockiasamy, S. et al. (2021). Anther Culture in Jatropha curcas L.: A Tree Species. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2289. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1331-3_14
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DOI: https://doi.org/10.1007/978-1-0716-1331-3_14
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-1331-3
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