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PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations

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PDZ Mediated Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2256))

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Abstract

PDZ domains are small globular domains involved in protein–protein interactions. They participate in a wide range of critical cellular processes. These domains, very abundant in the human proteome, are widely studied by high-throughput interactomics approaches and by biophysical and structural methods. However, the quality of the results is strongly related to the optimal folding and solubility of the domains. We provide here a detailed description of protocols for a strict quality assessment of the PDZ constructs. We describe appropriate experimental approaches that have been selected to overcome the small size of such domains to check the purity, identity, homogeneity, stability, and folding of samples.

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Acknowledgments

The authors acknowledge Florence Cordier for technical support in NMR. The authors thank the molecular biophysics facility at Institut Pasteur for providing cutting-edge instruments.

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Author notes

  1. Célia Caillet-Saguy and Sébastien Brûlé contributed equally to this work.

Authors and Affiliations

  1. Institut Pasteur, Unité Récepteurs-Canaux, CNRS UMR 3571, Paris, France

    Célia Caillet-Saguy & Nicolas Wolff

  2. Institut Pasteur, Plate-forme de Biophysique Moléculaire, CNRS UMR 3528, Paris, France

    Sébastien Brûlé & Bertrand Raynal

Authors
  1. Célia Caillet-Saguy
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  2. Sébastien Brûlé
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  3. Nicolas Wolff
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  4. Bertrand Raynal
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Corresponding author

Correspondence to Nicolas Wolff .

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Editors and Affiliations

  1. Centre de Recherche en CancÕrologie de Marseille, Aix-Marseille University, Inserm, CNRS, Institut Paoli-Calmettes, Marseille, France

    Jean-Paul Borg

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Caillet-Saguy, C., Brûlé, S., Wolff, N., Raynal, B. (2021). PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations. In: Borg, JP. (eds) PDZ Mediated Interactions. Methods in Molecular Biology, vol 2256. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1166-1_6

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  • DOI: https://doi.org/10.1007/978-1-0716-1166-1_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1165-4

  • Online ISBN: 978-1-0716-1166-1

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