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Phenotypical Characterization and Isolation of Tumor-Derived Mouse Myeloid-Derived Suppressor Cells

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Myeloid-Derived Suppressor Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2236))

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Abstract

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous cell population composed of mature and immature cells of myeloid origin that play a major role in tumor progression by inhibiting the antitumor immune responses mediated by T cells. In this chapter, we describe protocols for isolation, phenotypical and functional evaluation of MDSCs isolated from mouse tumors, with the aim at unifying and standardizing protocols set up by different laboratories.

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Acknowledgments

This work is supported by grants to Vincenzo Bronte: Cancer Research Institute (Clinic and Laboratory Integration Program, CLIP_2017), Euronanomed III (Joint Traslational Call_2017, Project Resolve), Cariverona Fondation (Project call, 2017), Qatar National Priority Research Program 2017 (Project: NPRP11S-1211-170086) and PRIN program of Italian Ministry of Education, University and Research (MIUR) (CUP: BRD19000260006); and to Stefano Ugel:.PRIN Program of Italian Ministry of Education, University and Research (MIUR) (CUP: B38D19000140006) and Associazione Italiana per la Ricerca sul Cancro (AIRC, Project: 21509).

Author Contributions

All authors listed have made a substantial, direct, and intellectual contribution to the work.

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Author notes

  1. Roza Maria Barouni and Chiara Musiu contributed equally to this work.

Authors and Affiliations

  1. Department of Medicine, Section of Immunology, University of Verona, Verona, Italy

    Roza Maria Barouni, Chiara Musiu, Vincenzo Bronte, Stefano Ugel & Stefania Canè

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  1. Roza Maria Barouni
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  2. Chiara Musiu
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  3. Vincenzo Bronte
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  4. Stefano Ugel
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  5. Stefania Canè
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Corresponding author

Correspondence to Vincenzo Bronte .

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Editors and Affiliations

  1. Experimental and Translational Research, Department of Otorhinolaryngology, University Hospital Essen, West German Cancer Center, Essen, Germany

    Sven Brandau

  2. Institute of Immunology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany

    Anca Dorhoi

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Barouni, R.M., Musiu, C., Bronte, V., Ugel, S., Canè, S. (2021). Phenotypical Characterization and Isolation of Tumor-Derived Mouse Myeloid-Derived Suppressor Cells. In: Brandau, S., Dorhoi, A. (eds) Myeloid-Derived Suppressor Cells. Methods in Molecular Biology, vol 2236. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1060-2_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1060-2_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1059-6

  • Online ISBN: 978-1-0716-1060-2

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