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The Comprehensive and Reliable Detection of Secondary Metabolites in Trichoderma reesei: A Tool for the Discovery of Novel Substances

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Trichoderma reesei

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2234))

Abstract

A method based on reversed phase high-performance liquid chromatography coupled with electrospray ionization high-resolution mass spectrometry (RP-HPLC-ESI-HRMS) for the comprehensive and reliable detection of secondary metabolites of Trichoderma reesei cultured in synthetic minimal liquid medium is presented. A stable isotope-assisted (SIA) workflow is used, which allows the automated, comprehensive extraction of truly fungal metabolite-derived LC-MS signals from the acquired chromatographic data. The subsequent statistical data analysis and a typical outcome of such a metabolomics data evaluation are shown by way of example in a previously published study on the influence of the pleiotropic regulator transcription factor Xylanase promoter binding protein 1 (Xpp1) in T. reesei on secondary metabolism.

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Acknowledgments

We thank Christian Derntl, Astrid R. Mach-Aigner, Robert L. Mach, and Bernhard Kluger very much for providing the Xpp1 dataset, which has been used to exemplify data evaluation in this protocol. Further the financial support by the Austrian Science Fund (FWF project P 29556-B22) is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Agrobiotechnology (IFA-Tulln), Institute of Bioanalytics and Agro-Metabolomics, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria

    Bernhard Seidl, Christoph Bueschl & Rainer Schuhmacher

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  1. Bernhard Seidl
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  2. Christoph Bueschl
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  3. Rainer Schuhmacher
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Correspondence to Rainer Schuhmacher .

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Editors and Affiliations

  1. Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

    Astrid R. Mach-Aigner

  2. Christian Doppler Laboratory for Optimized Expression of Carbohydrate-Active Enzymes, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

    Roland Martzy

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Seidl, B., Bueschl, C., Schuhmacher, R. (2021). The Comprehensive and Reliable Detection of Secondary Metabolites in Trichoderma reesei: A Tool for the Discovery of Novel Substances. In: Mach-Aigner, A.R., Martzy, R. (eds) Trichoderma reesei. Methods in Molecular Biology, vol 2234. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1048-0_19

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  • DOI: https://doi.org/10.1007/978-1-0716-1048-0_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1047-3

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