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In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis

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Developmental Biology of the Sea Urchin and Other Marine Invertebrates

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2219))

Abstract

The Porifera are one of the best candidates as the sister group to all other metazoans. Studies on this phylum are therefore expected to shed light on the origin and early evolution of key animal features. Transcriptomic or genomic data acquired during the last 10 years have highlighted the conservation of most of the main genes and pathways involved in the development of the other metazoans. The next step is to determine how similar genetic tool boxes can result in widely dissimilar body plan organization, dynamics, and life histories. To answer these questions, three main axes of research are necessary: (1) conducting more gene expression studies; (2) developing knockdown protocols; and (3) reinterpreting sponge cell biology using modern tools. In this chapter we focus on the in situ hybridization (ISH) technique, needed to establish the spatiotemporal expression of genes, both on whole mount individuals and paraffin sections, and at different stages of development (adults, embryos, larvae, buds) of the homoscleromorph sponge Oscarella lobularis.

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Author information

Author notes

  1. Laura Fierro-Constaín and Caroline Rocher contributed equally to this work.Emmanuelle Renard and Carole Borchiellini equally supervised this work.

Authors and Affiliations

  1. Department of Genetics and Evolution, Faculty of Sciences, Institute of Genetics and Genomics in Geneva (IGe3), University of Geneva, Geneva, Switzerland

    Quentin Schenkelaars

  2. Aix Marseille Univ, Avignon Université, CNRS, IRD, IMBE, Marseille, France

    Laura Fierro-Constaín, Caroline Rocher, Florent Marschal, Quentin Schenkelaars, Nina Séjourné, Carole Borchiellini & Emmanuelle Renard

Authors
  1. Laura Fierro-Constaín
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  2. Caroline Rocher
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  3. Florent Marschal
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  4. Quentin Schenkelaars
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  5. Nina Séjourné
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  6. Carole Borchiellini
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  7. Emmanuelle Renard
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Corresponding authors

Correspondence to Carole Borchiellini or Emmanuelle Renard .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, Midwestern University, Glendale, AZ, USA

    David J. Carroll

  2. Department of Biology, University of New Mexico, Albuquerque, NM, USA

    Stephen A. Stricker

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Fierro-Constaín, L. et al. (2021). In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis. In: Carroll, D.J., Stricker, S.A. (eds) Developmental Biology of the Sea Urchin and Other Marine Invertebrates. Methods in Molecular Biology, vol 2219. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0974-3_11

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  • DOI: https://doi.org/10.1007/978-1-0716-0974-3_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0973-6

  • Online ISBN: 978-1-0716-0974-3

  • eBook Packages: Springer Protocols

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