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Ex Vivo Culture for Preimplantation Mouse Embryo to Analyze Pluripotency

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Epigenetic Reprogramming During Mouse Embryogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2214))

Abstract

A couple of days after fertilization of a mouse oocyte by a sperm, two sequential cell differentiation events segregate pluripotent cells that can be identified by the presence of specific markers. Early mammalian embryos are relatively easy to recover as they are not yet implanted in the uterus matrix. Several decades of experimentation have enabled to find appropriate media to culture them, and therefore provide an excellent way to test different experimental setups such as the use of signaling inhibitors. We provide here a commonly used protocol to culture preimplantation embryos as well as a method to detect pluripotent cells in blastocysts.

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Authors and Affiliations

  1. Institut GReD, Université Clermont Auvergne, CNRS, Inserm, Clermont-Ferrand, 63000, France

    Katia Boutourlinsky, Nicolas Allègre & Claire Chazaud

Authors
  1. Katia Boutourlinsky
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  2. Nicolas Allègre
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  3. Claire Chazaud
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Corresponding author

Correspondence to Claire Chazaud .

Editor information

Editors and Affiliations

  1. Institut Curie, CNRS UMR3215/ INSERM U934, Paris Sciences & Lettres Research University (PSL), Paris, France

    Katia Ancelin

  2. Institut Curie, CNRS UMR3215/ INSERM U934, Paris Sciences & Lettres Research University (PSL), Institut de Ge´ne´tique Mole´culaire de Montpellier, Univ Montpellier, CNRS Montpellier, France, Paris, France

    Maud Borensztein

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Boutourlinsky, K., Allègre, N., Chazaud, C. (2021). Ex Vivo Culture for Preimplantation Mouse Embryo to Analyze Pluripotency. In: Ancelin, K., Borensztein, M. (eds) Epigenetic Reprogramming During Mouse Embryogenesis. Methods in Molecular Biology, vol 2214. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0958-3_1

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  • DOI: https://doi.org/10.1007/978-1-0716-0958-3_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0957-6

  • Online ISBN: 978-1-0716-0958-3

  • eBook Packages: Springer Protocols

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