Abstract
Key developmental processes of cell fate decisions and morphogenetic transformations take place during the periimplantation and early postimplantation stages of mouse embryogenesis. However analysing these fundamental events relies on direct observations of cultured embryos, which are challenging to obtain. To address this challenge, here we provide a detailed protocol describing a workflow for isolating early implanted embryos, removing of redundant extraembryonic tissues and describing the culture conditions that support further embryo development in vitro.
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References
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Acknowledgments
This work was supported by the German Research Foundation (DFG) Emmy Noether grant to I.B. and the Cells-in-Motion Cluster of Excellence/International Max Planck Research School (CiM/IMPRS) Ph.D. fellowship of N.G.
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Govindasamy, N., Bedzhov, I. (2019). Isolation and Culture of Periimplantation and Early Postimplantation Mouse Embryos. In: Herrick, J. (eds) Comparative Embryo Culture. Methods in Molecular Biology, vol 2006. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9566-0_25
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DOI: https://doi.org/10.1007/978-1-4939-9566-0_25
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9565-3
Online ISBN: 978-1-4939-9566-0
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