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Extraction of Small RNAs by Titanium Dioxide Nanofibers

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RNA Abundance Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2170))

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Abstract

MicroRNAs (miRNAs) are small RNAs, that bind to mRNA targets and regulate their translation. Functional study of miRNAs and exploration of their utility as disease markers require miRNA extraction from biological samples, which contain large amounts of interfering compounds for downstream RNA identification and quantification. The most common extraction methods employ either silica columns or TRIzol reagent, but these approaches afford low recovery for small RNAs, possibly due to their short strand lengths. Here, we describe the fabrication of titanium dioxide nanofibers and the optimal extraction conditions to improve miRNA recovery from biological buffers, cell lysate, and serum.

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Author information

Authors and Affiliations

  1. Program in Biomedical Sciences, University of California, Riverside, CA, USA

    Luis A. Jimenez

  2. Department of Chemistry, University of California, Riverside, CA, USA

    Wenwan Zhong

Authors
  1. Luis A. Jimenez
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  2. Wenwan Zhong
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Corresponding author

Correspondence to Wenwan Zhong .

Editor information

Editors and Affiliations

  1. Department of Plant Pathology and Microbiology, Center for Plant Cell Biology and Institute for Integrative Genome Biology, University of California, Riverside, CA, USA

    Hailing Jin

  2. Department of Nematology, University of California, Riverside, CA, USA

    Isgouhi Kaloshian

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Jimenez, L.A., Zhong, W. (2021). Extraction of Small RNAs by Titanium Dioxide Nanofibers. In: Jin, H., Kaloshian, I. (eds) RNA Abundance Analysis . Methods in Molecular Biology, vol 2170. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0743-5_8

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  • DOI: https://doi.org/10.1007/978-1-0716-0743-5_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0742-8

  • Online ISBN: 978-1-0716-0743-5

  • eBook Packages: Springer Protocols

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