Abstract
The use of ex vivo compound action potential (CAP) recordings from intact optic nerves is an ideal model to study white matter function without the influence of gray matter. Here, we describe how freshly dissected optic nerves are placed in a humidified recording chamber and how evoked CAPs are recorded and monitored in real time for up to 10 h. Evoked CAP recordings allow for white matter to be studied under acute challenges such as anoxia, hypoxia, aglycemia, and ischemia.
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Acknowledgments
This work was supported by grants from National Institute on Aging (NIA) to Selva Baltan and NINDS to Sylvain Brunet and Selva Baltan, as well as a gift from Rose Mary Kubik. Selva Baltan has previously published as Selva Tekkök. The authors thank Dr. Chris Nelson, medical writer, for his help in editing this chapter. Materials and data were provided by the Cleveland Clinic Foundation (CCF). All rights, title, and interest in the materials and data are owned by the CCF.
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Bastian, C., Brunet, S., Baltan, S. (2020). Ex Vivo Studies of Optic Nerve Axon Electrophysiology. In: Babetto, E. (eds) Axon Degeneration. Methods in Molecular Biology, vol 2143. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0585-1_13
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DOI: https://doi.org/10.1007/978-1-0716-0585-1_13
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