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Extrusion-Based Bioprinting: Current Standards and Relevancy for Human-Sized Tissue Fabrication

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3D Bioprinting

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2140))

Abstract

The field of bioengineering has long pursued the goal of fabricating large-scale tissue constructs for use both in vitro and in vivo. Recent technological advances have indicated that bioprinting will be a key technique in manufacturing these specimens. This chapter aims to provide an overview of what has been achieved to date through the use of microextrusion bioprinters and what major challenges still impede progress. Microextrusion printer configurations will be addressed along with critical design characteristics including nozzle specifications and bioink modifications. Significant challenges within the field with regard to achieving long-term cell viability and vascularization, and current research that shows promise in mitigating these challenges in the near future are discussed. While microextrusion is a broad field with many applications, this chapter aims to provide an overview of the field with a focus on its applications toward human-sized tissue constructs.

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Authors and Affiliations

  1. Wake Forest Institute for Regenerative Medicine, Wake Forest University, Winston-Salem, NC, USA

    Kelsey Willson, Dongxu Ke, Carlos Kengla, Anthony Atala & Sean V. Murphy

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  1. Kelsey Willson
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  2. Dongxu Ke
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  3. Carlos Kengla
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  4. Anthony Atala
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  5. Sean V. Murphy
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Correspondence to Sean V. Murphy .

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  1. ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Squires Way, Fairy Meadow, NSW, Australia

    Jeremy M. Crook

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Willson, K., Ke, D., Kengla, C., Atala, A., Murphy, S.V. (2020). Extrusion-Based Bioprinting: Current Standards and Relevancy for Human-Sized Tissue Fabrication. In: Crook, J.M. (eds) 3D Bioprinting. Methods in Molecular Biology, vol 2140. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0520-2_5

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  • DOI: https://doi.org/10.1007/978-1-0716-0520-2_5

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