Abstract
The FUCCI indicator system is a powerful tool for spatio-temporal analysis of the cell cycle, but its utility has been restricted so far to a limited range of applications. Here, we describe how to establish and validate the FUCCI system in murine pluripotent stem cells (PSCs) and describe the utility of transgenic FUCCI mice. We then describe how the FUCCI system can be used to generate material for a wide-range of omics-based applications in conjunction with FACS isolation of cells. This significantly broadens the potential applications of FUCCI reporters for studying the molecular basis of development and disease.
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Acknowledgements
This work was supported by grants to S.D. from the National Institute of Child Health and Human Development (HD049647) and the National Institute for General Medical Sciences (GM75334). We thank Julie Nelson for assistance with FACS.
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Chappell, J., Boward, B., Dalton, S. (2015). Expanding the Utility of FUCCI Reporters Using FACS-Based Omics Analysis. In: Turksen, K. (eds) Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 1341. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_214
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DOI: https://doi.org/10.1007/7651_2015_214
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2953-5
Online ISBN: 978-1-4939-2954-2
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