Embryonic Stem Cell Protocols

  • Kursad Turksen

Part of the Methods in Molecular Biology book series (MIMB, volume 1341)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Keitaro Imaizumi, Momoe Iha, Naoki Nishishita, Shin Kawamata, Shinichi Nishikawa, Teruo Akuta
    Pages 15-24
  3. Marielle Afanassieff, Pierre Osteil, Pierre Savatier
    Pages 49-66
  4. Ortwin Naujok, Ulf Diekmann, Matthias Elsner
    Pages 67-85
  5. Anna Pezzarossa, Ana M. V. Guedes, Domingos Henrique, Elsa Abranches
    Pages 87-100
  6. James Chappell, Ben Boward, Stephen Dalton
    Pages 101-110
  7. Lida Radan, Chris S. Hughes, Jonathan H. Teichroeb, Lynne-Marie Postovit, Dean H. Betts
    Pages 133-142
  8. Anisa B. Rahim, Leah A. Vardy
    Pages 143-155
  9. Talita Glaser, Ana Regina G. Castillo, Ágatha Oliveira, Henning Ulrich
    Pages 245-255

About this book


This extensive volume explores areas of intense activity related to the very early commitment of stem cells to particular lineages and the progression of differentiation to mature cell stages. Research on embryonic stem cells continues to move very quickly, thus the kinds of studies continue to expand and diversify, and methodologies are continuously being refined and improved, which this book reflects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and fully updated, Embryonic Stem Cell Protocols, Third Edition serves as an ideal reference for researchers investigating this rich area of study.


Animal models Differentiation ESCs Pluripotent stem cells Stem cell lineage

Editors and affiliations

  • Kursad Turksen
    • 1
  1. 1.Ottawa Hospital Research InstituteOttawaCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2954-2
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2953-5
  • Online ISBN 978-1-4939-2954-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book