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Enhancing Induced Pluripotent Stem Cell Generation by MicroRNA

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Induced Pluripotent Stem (iPS) Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1357))

Abstract

Somatic reprogramming to generate induced pluripotent stem cells, or iPSC, is a powerful tool in developmental biology, disease modeling, and regenerative medicine. microRNAs have been shown to regulate many key pathways in iPSC induction. Here we describe a microRNA mimic enhanced somatic reprogramming process starting from mouse embryonic fibroblast isolation to iPSC induction to colony derivation and characterization.

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Authors and Affiliations

  1. Department of Bioengineering, Department of Pediatrics, University of California San Diego School of Medicine, 9500 Gilman Drive MC 0762, La Jolla, CA, 92093, USA

    Jason Dang

  2. Department of Pediatrics, University of California San Diego School of Medicine, 9500 Gilman Drive MC 0762, La Jolla, CA, 92093, USA

    Tariq M Rana

Authors
  1. Jason Dang
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  2. Tariq M Rana
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Corresponding author

Correspondence to Tariq M Rana .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

  2. Mount Sinai Hospital Samuel Lunenfeld Research Inst, Toronto, Ontario, Canada

    Andras Nagy

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© 2015 Springer Science+Business Media New York

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Dang, J., Rana, T.M. (2015). Enhancing Induced Pluripotent Stem Cell Generation by MicroRNA. In: Turksen, K., Nagy, A. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 1357. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_201

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  • DOI: https://doi.org/10.1007/7651_2015_201

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3054-8

  • Online ISBN: 978-1-4939-3055-5

  • eBook Packages: Springer Protocols

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