Abstract
iPS cells are attractive for the regenerative medicine. The creation of pluripotent cells from somatic cells has great potential for basic and clinical research and application. Retroviral transduction of four or three transfection factors has been shown to initiate a reprogramming process. Here, we describe the effect of transcription factors regarding the growth and differentiation of mouse iPS cells in normoxia or hypoxia. Furthermore, we introduce the function of hypoxia-inducible factors (HIFs) in mouse iPS cells in hypoxia using RT-PCR and western blotting together with HIFs knockdown techniques.
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Acknowledgements
This work was supported by Grants-in-Aid for Scientific Research (B) from Japan Society for The Promotion of Science, Contract Grant Number 22390360 to Y.H.
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Sugimoto, K., Hayashi, Y. (2014). The Characteristics of Murine iPS Cells and siRNA Transfection Under Hypoxia. In: Turksen, K., Nagy, A. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 1357. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_158
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DOI: https://doi.org/10.1007/7651_2014_158
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