Abstract
In vivo lineage tracing is a valuable technique to study cellular behavior. Our lab developed a lineage tracing method, based on the Cre/lox system, to genetically induce clonal labelling of cells and follow their progeny. Here we describe a protocol for temporally controlled clonal labelling and for microdissection of individual mouse hair follicles. We further present staining and visualization techniques used in our lab to analyze clones issued from genetically induced labelling.
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Acknowledgments
This work was supported by the Institut Pasteur and by the French Agence National de la Recherche n°10-01 (DEV-Process to I.S. and J.F.N). All experiments were carried out in accordance with the national guidelines for care and use of laboratory animals.
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Sequeira, I., Legué, E., Capgras, S., Nicolas, JF. (2013). Microdissection and Visualization of Individual Hair Follicles for Lineage Tracing Studies. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 1195. Springer, New York, NY. https://doi.org/10.1007/7651_2013_48
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DOI: https://doi.org/10.1007/7651_2013_48
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Print ISBN: 978-1-4939-1223-0
Online ISBN: 978-1-4939-1224-7
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