High Activity Preparations of Lipases and Proteases for Catalysis in Low Water Containing Organic Solvents and Ionic Liquids

  • Ipsita Roy
  • Joyeeta Mukherjee
  • Munishwar Nath Gupta
Part of the Methods in Molecular Biology book series (MIMB, volume 1051)


Simple precipitation of enzymes has shown impressive catalytic efficiencies in organic solvents. In asmuch as these can be recovered after the reaction, these can be viewed as immobilized preparations just like more extensively used cross-linked enzyme aggregates (CLEAs). This chapter describes three protocols which use these enzyme precipitated and rinsed with propanol/some other appropriate organic solvent. The first two protocols show their applications in ionic liquids for a transesterification reaction and a kinetic resolution. The third protocol presumably incorporates an “imprinting” effect so that the precipitates are now able to efficiently catalyze transesterification of tributyrin with tertiary alcohols.

Key words

Enzymes in organic solvent Enzymes in ionic liquids Kinetic resolution by enzymes Transesterification reactions Enzyme precipitation Lipases Proteases Biotransformation with tertiary alcohols Enzyme imprinting 



The authors thank Dr. Shweta Shah and Dr. Abir B. Majumder for the work described in this chapter. Financial support from the Department of Science and Technology (DST-SERB) [Grant No.: SR/SO/BB-68/2010], the Department of Biotechnology (DBT) [Grant No: BT/PR14103/BRB/10/808/2010], and Council for Scientific and Industrial Research (CSIR), all Government of India organizations, is gratefully acknowledged.


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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Ipsita Roy
    • 1
  • Joyeeta Mukherjee
    • 2
  • Munishwar Nath Gupta
    • 2
  1. 1.Department of BiotechnologyNational Institute of Pharmaceutical Education and ResearchPunjabIndia
  2. 2.Department of ChemistryIndian Institute of Technology DelhiNew DelhiIndia

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