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The synthesis of a D-amino acid ester in an organic media with α-chymotrypsin modified by a bio-imprinting procedure

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Summary

In this report the use of chymotrypsin in the synthesis of a D-amino acid ester is described. The enzyme was modified to accept the D-form of a tryptophane derivative, by precipitating the enzyme-inhibitor complex between chymotrypsin and N-acetyl-D-tryptophane in 1-propanol. The precipitate was then used to synthesize N-acetyl-D-tryptophane ethyl ester in cyclhexane. The rate of the D-esterification was 7.5 nmole/mg enzyme·h.

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Authors and Affiliations

  1. Pure and Applied Biochemistry, Chemical Center, P.O.B.124, S-221 00, Lund, Sweden

    Marianne Ståhl, Mats-Olle Månsson & Klaus Mosbach

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  1. Marianne Ståhl
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  2. Mats-Olle Månsson
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  3. Klaus Mosbach
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Ståhl, M., Månsson, MO. & Mosbach, K. The synthesis of a D-amino acid ester in an organic media with α-chymotrypsin modified by a bio-imprinting procedure. Biotechnol Lett 12, 161–166 (1990). https://doi.org/10.1007/BF01026792

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  • DOI: https://doi.org/10.1007/BF01026792

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