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Monitoring β-Arrestin 2 Targeting to the Centrosome, Basal Body, and Primary Cilium by Fluorescence Microscopy

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Beta-Arrestins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1957))

Abstract

Primary cilia (PC) are microtubule-based organelles that behave like a cellular antenna controlling key signaling pathways during development and tissue homeostasis. The ciliary membrane is highly enriched for G protein-coupled receptors (GPCRs), and PC are a crucial signaling compartment for this large receptor family. Downstream effectors of GPCR signaling are also present in cilia, and evidence obtained by our labs and others demonstrated that β-arrestin (βarr) family members are differentially recruited to PC and have investigated the role of GPCR activation in this process. In this chapter, we provide methods based on fluorescence microscopy on fixed or live cells suitable for investigating targeting and recruitment of βarrs at PC.

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Author information

Authors and Affiliations

  1. Department of Genetics and Developmental Biology, Institut Curie, CNRS-UMR3215, INSERM-U934, Paris, France

    Anahi Molla-Herman

  2. CIRB, Collège de France, CNRS-UMR7241, INSERM-U1050, Paris, France

    Anahi Molla-Herman

  3. Department of Biological Chemistry and Pharmacology, The Ohio State University, Columbus, OH, USA

    Kathryn M. Davis & Kirk Mykytyn

  4. Neuroscience Research Institute, The Ohio State University, Columbus, OH, USA

    Kirk Mykytyn

  5. Laboratoire des Maladies Rénales Héréditaires, INSERM U1163, Paris, France

    Alexandre Benmerah

  6. Institut Imagine, Université Paris Descartes, Sorbonne Paris Cité, Paris, France

    Alexandre Benmerah

Authors
  1. Anahi Molla-Herman
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  2. Kathryn M. Davis
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  3. Kirk Mykytyn
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  4. Alexandre Benmerah
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Corresponding authors

Correspondence to Anahi Molla-Herman , Kirk Mykytyn or Alexandre Benmerah .

Editor information

Editors and Affiliations

  1. Institut Cochin, INSERM U1016, Paris, France

    Mark G. H. Scott

  2. Department of Medicine, Research Institute of the McGill, University Health Center (RI-MUHC), McGill University, Montreal, QC, Canada

    Stéphane A. Laporte

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Molla-Herman, A., Davis, K.M., Mykytyn, K., Benmerah, A. (2019). Monitoring β-Arrestin 2 Targeting to the Centrosome, Basal Body, and Primary Cilium by Fluorescence Microscopy. In: Scott, M., Laporte, S. (eds) Beta-Arrestins. Methods in Molecular Biology, vol 1957. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9158-7_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9158-7_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9157-0

  • Online ISBN: 978-1-4939-9158-7

  • eBook Packages: Springer Protocols

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