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Preparation of Pulmonary Artery Myocytes and Rings to Study Vasoactive GPCRs

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G Protein-Coupled Receptor Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1947))

Abstract

G protein-coupled receptors (GPCR) are crucial transducers of extracellular signals into changes in vascular tone. Vasoactive GPCR stimulation in the pulmonary circuit may be elicited by agonists released in acute tissue hypoxia or inflammation, as well as chronic disease. Acute responses involve activation of smooth muscle contraction or relaxation machinery causing changes in actomyosin interaction, thereby altering lumen diameter. Chronic responses may typically include activation of proliferation or fibrosis. Using pulmonary artery myocytes and pulmonary artery rings, we describe a general strategy for quantification of vasoconstrictor or vasodilator GPCR responses, and for comparison of signaling pathways in cultured cells and in contracted vessels using immunohistochemistry of contracting vessels.

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Acknowledgments

Supported by a grant-in-aid from Heart and Stroke Foundation and a mid-career operating grant from Research Manitoba (SD).

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Authors and Affiliations

  1. Biology of Breathing Group, Children’s Hospital Research Institute of Manitoba, Winnipeg, MB, Canada

    Martha Hinton, Anurag Singh Sikarwar & Shyamala Dakshinamurti

  2. Department of Oral Biology, University of Manitoba, Winnipeg, MB, Canada

    Anurag Singh Sikarwar

  3. Department of Physiology, University of Manitoba, Winnipeg, MB, Canada

    Shyamala Dakshinamurti

  4. Departments of Pediatrics, University of Manitoba, Winnipeg, MB, Canada

    Shyamala Dakshinamurti

Authors
  1. Martha Hinton
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  2. Anurag Singh Sikarwar
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  3. Shyamala Dakshinamurti
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Corresponding author

Correspondence to Shyamala Dakshinamurti .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute (Neurosciences), Ottawa, ON, Canada

    Mario Tiberi

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Hinton, M., Sikarwar, A.S., Dakshinamurti, S. (2019). Preparation of Pulmonary Artery Myocytes and Rings to Study Vasoactive GPCRs. In: Tiberi, M. (eds) G Protein-Coupled Receptor Signaling. Methods in Molecular Biology, vol 1947. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9121-1_23

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  • DOI: https://doi.org/10.1007/978-1-4939-9121-1_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9120-4

  • Online ISBN: 978-1-4939-9121-1

  • eBook Packages: Springer Protocols

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