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Preparation of Pulmonary Artery Myocytes and Rings to Study Vasoactive GPCRs

  • Martha Hinton
  • Anurag Singh Sikarwar
  • Shyamala DakshinamurtiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1947)

Abstract

G protein-coupled receptors (GPCR) are crucial transducers of extracellular signals into changes in vascular tone. Vasoactive GPCR stimulation in the pulmonary circuit may be elicited by agonists released in acute tissue hypoxia or inflammation, as well as chronic disease. Acute responses involve activation of smooth muscle contraction or relaxation machinery causing changes in actomyosin interaction, thereby altering lumen diameter. Chronic responses may typically include activation of proliferation or fibrosis. Using pulmonary artery myocytes and pulmonary artery rings, we describe a general strategy for quantification of vasoconstrictor or vasodilator GPCR responses, and for comparison of signaling pathways in cultured cells and in contracted vessels using immunohistochemistry of contracting vessels.

Key words

GPCR Pulmonary artery Smooth muscle cells Vasoconstriction Myography 

Notes

Acknowledgments

Supported by a grant-in-aid from Heart and Stroke Foundation and a mid-career operating grant from Research Manitoba (SD).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Martha Hinton
    • 1
  • Anurag Singh Sikarwar
    • 1
    • 2
  • Shyamala Dakshinamurti
    • 1
    • 3
    • 4
    Email author
  1. 1.Biology of Breathing GroupChildren’s Hospital Research Institute of ManitobaWinnipegCanada
  2. 2.Department of Oral BiologyUniversity of ManitobaWinnipegCanada
  3. 3.Department of PhysiologyUniversity of ManitobaWinnipegCanada
  4. 4.Departments of PediatricsUniversity of ManitobaWinnipegCanada

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