G Protein-Coupled Receptor Signaling

Methods and Protocols

  • Mario Tiberi

Part of the Methods in Molecular Biology book series (MIMB, volume 1947)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Probing Structural Properties of GPCRs

    1. Front Matter
      Pages 1-1
    2. Katsufumi Tomobe, Eiji Yamamoto, Kenji Yasuoka
      Pages 21-30
    3. Anne Zemella, Theresa Richter, Lena Thoring, Stefan Kubick
      Pages 57-77 Open Access
  3. Tweaking Ligands for GPCR Studies

    1. Front Matter
      Pages 79-79
    2. Marleen Van Troys, Willem Vannecke, Christophe Ampe, Annemieke Madder
      Pages 81-102
    3. Amanda Acosta-Ruiz, Johannes Broichhagen, Joshua Levitz
      Pages 103-136
    4. Sridévi M. Ramanoudjame, Lucie Esteoulle, Stéphanie Riché, Jean-François Margathe, Thierry Durroux, Iuliia A. Karpenko et al.
      Pages 137-147
  4. Bioluminescence and Fluorescence Resonance Transfer Energy Approaches

    1. Front Matter
      Pages 149-149
    2. Joyce Heuninck, Candide Hounsou, Elodie Dupuis, Eric Trinquet, Bernard Mouillac, Jean-Philippe Pin et al.
      Pages 151-168
    3. Kyla Bourque, Dominic Devost, Asuka Inoue, Terence E. Hébert
      Pages 169-182
    4. Amina M. Bagher, Melanie E. M. Kelly, Eileen M. Denovan-Wright
      Pages 199-215
  5. Exploring GPCR Signaling Properties

    1. Front Matter
      Pages 239-239
    2. Geneviève Laroche, Patrick M. Giguère
      Pages 257-267
    3. Burcu Hasdemir, Juan A. Oses-Prieto, Alma Burlingame, Aditi Bhargava
      Pages 269-285
  6. Visualizing Subcellular Compartmentalization of GPCRs

    1. Front Matter
      Pages 287-287
    2. Samuel Génier, Jade Degrandmaison, Christine L. Lavoie, Louis Gendron, Jean-Luc Parent
      Pages 289-302
    3. Xuezhi Li, Stéphanie Rosciglione, Andréanne Laniel, Christine Lavoie
      Pages 303-322
    4. Fabiana A. Caetano Crowley, Bryan Heit, Stephen S. G. Ferguson
      Pages 323-336
  7. Investigating In Vivo Functions of GPCRs

    1. Front Matter
      Pages 349-349
    2. Marc López-Cano, Joan Font, Amadeu Llebaria, Víctor Fernández-Dueñas, Francisco Ciruela
      Pages 351-359
    3. Dmitry Akhmedov, Nicholas S. Kirkby, Jane A. Mitchell, Rebecca Berdeaux
      Pages 361-376
    4. Martha Hinton, Anurag Singh Sikarwar, Shyamala Dakshinamurti
      Pages 389-401
  8. Back Matter
    Pages 403-406

About this book


This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs. Sections explore the tweaking of ligands, bioluminescence and FRET approaches, specific GPCR signaling properties, as well as visualization of subcellular compartmentalization. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, G Protein-Coupled Receptor Signaling: Methods and Protocols serves as an ideal reference for life scientists working in a variety of research fields including molecular pharmacology, cell and developmental biology, brain behavior and physiology, drug development and screening.

Chapter  4 is available open access under a CC BY 4.0 license via


Transmembrane protein families Molecular pharmacology Brain physiology Drug development Developmental biology GPCR drugs

Editors and affiliations

  • Mario Tiberi
    • 1
  1. 1.Ottawa Hospital Research Institute (Neurosciences)OttawaCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9120-4
  • Online ISBN 978-1-4939-9121-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site