Quantification of various proteins expressed in yeast can be performed by different methods. In this respect, classical as well as advanced techniques can be applied, where the analysis of crude supernatants is of special interest in screening but also manufacturing.
The following chapter addresses the analytical background of the introduced methods followed by specific recommendations for the quantification of different products of industrial interest. The method portfolio includes electrophoresis, chromatography, and ELISA as classical techniques, but also biosensor-based, microfluidic and automated, miniaturized methods are introduced. Furthermore, individual strengths and perceived limitations are summarized.
Although prominent examples are described, it should be noted that individual modifications are required according to host and cultivation mode.
Protein quantification Electrophoresis HPLC ELISA Bio-layer interferometry Microfluidics CD technology Surface plasmon resonance Electrochemiluminescence assays Microchip-based assays
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