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Protein Stains and Applications

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Protein Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 869))

Abstract

Staining of proteins separated on gels provides the basis for determination of the critical properties of these biopolymers, such as their molecular weight and/or charge. Detection of proteins on gels and blots require stains. These stains vary in sensitivity, ease of use, color, stability, versatility, and specificity. This review discusses different stains and applications with details on how to use the advantages and disadvantages of each stain. It also compiles some important points to be considered in imaging and evaluation. Commonly used colorimetric and fluorescent dyes for general protein staining, and posttranslational modification-specific detection methods are also discussed.

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Author information

Authors and Affiliations

  1. Recombinant Technologies LLC, Cheshire, CT, USA

    Ranjini K. Sundaram & Pazhani Sundaram

  2. University of Colorado Denver, Aurora, CO, USA

    Natarajan Balasubramaniyan

Authors
  1. Ranjini K. Sundaram
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  2. Natarajan Balasubramaniyan
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  3. Pazhani Sundaram
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Editor information

Editors and Affiliations

  1. Oklahoma Medical Research Foundation, Univ. of Oklahoma Health Sciences Center, NE. 13th Street 825, Oklahoma City, 73104, Oklahoma, USA

    Biji T. Kurien

  2. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, Oklahoma, USA

    R. Hal Scofield

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© 2012 Springer Science+Business Media, LLC

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Sundaram, R.K., Balasubramaniyan, N., Sundaram, P. (2012). Protein Stains and Applications. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_39

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  • DOI: https://doi.org/10.1007/978-1-61779-821-4_39

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-820-7

  • Online ISBN: 978-1-61779-821-4

  • eBook Packages: Springer Protocols

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