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Chromatin Immunoprecipitation (ChiP) Protocol for the Analysis of Gene Regulation by Histone Modifications in Agave angustifolia Haw

  • Rosa Us-Camas
  • Clelia De-la-Peña
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1815)

Abstract

Chromatin is a dynamic entity that regulates different biological processes crucial for the proper functioning of the cell. Chromatin regulation depends largely on the interactions that occur between DNA with histones and nonhistone proteins. The chromatin immunoprecipitation assay (ChiP) is a widely used technique for the study of these DNA-histone and DNA-nonhistone interactions and their biological repercussions. Here we describe a ChiP protocol that allows in vivo analysis of the associations of histone modifications with genomic DNA in Agave angustifolia Haw. Although this protocol is established for A. angustifolia, it can be used in other species to obtain similar results. We also propose a strategy to shorten the times in some steps of the standard protocol.

Key words

Agave angustifolia ChiP H3K4me3 H3K27me3 LCYβ PEPCase RubS 

Notes

Acknowledgments

The work from CDLP laboratory was supported by a grant received from the National Council for Science and Technology (CONACyT, 1515).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Unidad de Biotecnología, Centro de Investigación Científica de YucatánMéridaMexico

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