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Chromatin Immunoprecipitation (ChiP) Protocol for the Analysis of Gene Regulation by Histone Modifications in Agave angustifolia Haw

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Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1815))

Abstract

Chromatin is a dynamic entity that regulates different biological processes crucial for the proper functioning of the cell. Chromatin regulation depends largely on the interactions that occur between DNA with histones and nonhistone proteins. The chromatin immunoprecipitation assay (ChiP) is a widely used technique for the study of these DNA-histone and DNA-nonhistone interactions and their biological repercussions. Here we describe a ChiP protocol that allows in vivo analysis of the associations of histone modifications with genomic DNA in Agave angustifolia Haw. Although this protocol is established for A. angustifolia, it can be used in other species to obtain similar results. We also propose a strategy to shorten the times in some steps of the standard protocol.

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Acknowledgments

The work from CDLP laboratory was supported by a grant received from the National Council for Science and Technology (CONACyT, 1515).

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Authors and Affiliations

  1. Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 #130 x 32 y 34, Col.Chuburná de Hidalgo, C.P. 97205, Mérida, Yucatán, Mexico

    Rosa Us-Camas & Clelia De-la-Peña

Authors
  1. Rosa Us-Camas
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  2. Clelia De-la-Peña
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Corresponding author

Correspondence to Clelia De-la-Peña .

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Editors and Affiliations

  1. Centro de Investigación Científica de Yucatán, Unidad de Bioquímica y Biología, Molecular de Plantas, Mérida, Yucatán, Mexico

    Víctor M. Loyola-Vargas

  2. Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, Mexico

    Neftalí Ochoa-Alejo

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Us-Camas, R., De-la-Peña, C. (2018). Chromatin Immunoprecipitation (ChiP) Protocol for the Analysis of Gene Regulation by Histone Modifications in Agave angustifolia Haw. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 1815. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8594-4_26

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  • DOI: https://doi.org/10.1007/978-1-4939-8594-4_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8593-7

  • Online ISBN: 978-1-4939-8594-4

  • eBook Packages: Springer Protocols

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