Mitochondria pp 293-314 | Cite as

Analysis of Yeast Mitochondria by Electron Microscopy

  • Ann-Katrin Unger
  • Stefan Geimer
  • Max Harner
  • Walter Neupert
  • Benedikt Westermann
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1567)

Abstract

Budding yeast Saccharomyces cerevisiae represents a widely used model organism for the study of mitochondrial biogenesis and architecture. Electron microscopy is an essential tool in the analysis of cellular ultrastructure and the precise localization of proteins to organellar subcompartments. We provide here detailed protocols for the analysis of yeast mitochondria by transmission electron microscopy: (1) chemical fixation and Epon embedding of yeast cells and isolated mitochondria, and (2) cryosectioning and immunolabeling of yeast cells and isolated mitochondria according to the Tokuyasu method.

Key words

Immunoelectron microscopy Mitochondria Organelle architecture Saccharomyces cerevisiae Tokuyasu cryosectioning Transmission electron microscopy 

Notes

Acknowledgments

We thank Fulvio Reggiori and Muriel Mari (University Medical Centre Utrecht, The Netherlands) for advice and excellent training in cryo-sectioning, Rita Grotjahn for technical assistance, and Anja Heublein for photography. This work was supported by the Elitenetzwerk Bayern and a Max-Planck-Fellowship to A.-K.U. and grants of the Deutsche Forschungsgemeinschaft to B.W.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Ann-Katrin Unger
    • 1
  • Stefan Geimer
    • 1
  • Max Harner
    • 2
  • Walter Neupert
    • 2
  • Benedikt Westermann
    • 1
  1. 1.Institut für ZellbiologieUniversität BayreuthBayreuthGermany
  2. 2.Max Planck Institut für BiochemieMartinsriedGermany

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