Non-Viral Gene Delivery Vectors pp 159-174

Part of the Methods in Molecular Biology book series (MIMB, volume 1445) | Cite as

Polyallylamine Derivatives: Novel NonToxic Transfection Agents

Protocol

Abstract

Cationic polymers have shown great potential for the delivery of proteins, nucleic acids forming complexes, called polyplexes. The most important issue in the context of using cationic polymers as carriers is the balance between the high transfection efficiency and low cytotoxicity. In this chapter, we report the preparation of polyallylamine derivatives mainly based on substitution of amino groups by glycidyltrimethylammonium chloride. The resulting polyplexes enhance the transfection of HeLa cell line without cytotoxic effects. Here, we describe methods for preparation and characterization of polyplexes using dynamic light scattering, ζ-potential measurements, gel retardation assay, and atomic force microscopy. Moreover, we provide protocols for the transfection of HeLa cell line by polyplexes, determination of their cytotoxicity, cell uptake, and intracellular trafficking.

Key words

Polymer Plasmid Polyplexes Transfection Gene delivery Nanocondensation Carriers 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Academic Centre for Materials and NanotechnologyAGH University of Science and TechnologyKrakowPoland
  2. 2.Department of Physical Chemistry and Electrochemistry, Faculty of ChemistryJagiellonian UniversityKrakówPoland
  3. 3.Centre de Biophysique MoléculaireUPR4301 CNRS affiliated to the University of OrléansOrléans Cedex 2France

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