Abstract
Recent advances in high-throughput sequencing have shed new light on the diversity of small noncoding RNA (sncRNA) classes and their crucial roles in gene regulation and disease. One key step in sncRNA profiling consists in their quantification and assessment of their degradation extent. In this chapter, we will describe different gold standard methods used to achieve both purposes before using the sncRNAs in downstream applications.
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Marchand, V., Ayadi, L., Bourguignon-Igel, V., Motorin, Y. (2021). Quantitative and Qualitative Assessment of Small RNA Preparations. In: Rederstorff, M. (eds) Small Non-Coding RNAs. Methods in Molecular Biology, vol 2300. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1386-3_3
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DOI: https://doi.org/10.1007/978-1-0716-1386-3_3
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1385-6
Online ISBN: 978-1-0716-1386-3
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