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Small Non-Coding RNAs

Methods and Protocols

  • Book
  • © 2021
  • Latest edition

Overview

Editors:
  1. Mathieu Rederstorff

    1. Université de Lorraine, CNRS, IMoPA, Nancy, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2300)

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About this book

This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology.  Chapters guide readers through customized dedicated protocols and technologies that will be of valuable help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on  troubleshooting and avoiding known pitfalls.

Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies.

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Introduction to Small Non-Coding RNAs

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Regulatory Non-Coding RNAs: An Overview

      • Camille Virciglio, Yoann Abel, Mathieu Rederstorff
      Pages 3-9

    3. RNA Precipitation

      • Valérie Bourguignon-Igel, Yoann Abel, Mathieu Rederstorff
      Pages 11-16

    4. Quantitative and Qualitative Assessment of Small RNA Preparations

      • Virginie Marchand, Lilia Ayadi, Valérie Bourguignon-Igel, Yuri Motorin
      Pages 17-29

    5. Non-Coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection

      • Charbel Alfeghaly, Christelle Aigueperse, Sylvain Maenner, Isabelle Behm-Ansmant
      Pages 31-37

  3. Detection of Small Non-Coding RNAs

    1. Front Matter

      Pages 39-39
      Download chapter PDF

    2. Northern Blot Detection of Tiny RNAs

      • Jana C. Wiegard, M. Amri C. Schlüter, Olga Y. Burenina, Elena A. Kubareva, Gabriele Klug, Arnold Grünweller et al.
      Pages 41-58

    3. Stem-Loop qRT-PCR–Based Quantification of miRNAs

      • Yoann Abel, Mathieu Rederstorff
      Pages 59-64

    4. Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation

      • Marlène Vayssières, Gabrielle Bourgeois, Florian Chardon, Anne-Sophie Tillault, Magali Blaud
      Pages 65-72

    5. Fluorescence In Situ Hybridization of Small Non-Coding RNAs

      • Valentin Vautrot, Géraud Heckler, Christelle Aigueperse, Isabelle Behm-Ansmant
      Pages 73-85

  4. Functional Analysis of Small Non-Coding RNAs

    1. Front Matter

      Pages 87-87
      Download chapter PDF

    2. Using Native RIP, UV-CLIP or fCLIP to Address Protein–RNA Interactions In Vivo

      • Laeya Baldini, Stéphane Labialle
      Pages 89-98

    3. Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography

      • Aymeric Sanchez, Sylvain Maenner
      Pages 99-106

    4. Study of Genome-Wide Occupancy of Long Non-Coding RNAs Using Chromatin Isolation by RNA Purification (ChIRP)

      • Charbel Alfeghaly, Isabelle Behm-Ansmant, Sylvain Maenner
      Pages 107-117

    5. Gene Reporter Assays to Study miRNA Function

      • Yoann Abel, Mathieu Rederstorff
      Pages 119-131

    6. CRISPR/Cas9 System to Knockdown MicroRNA In Vitro and In Vivo

      • Bin Yi, Kristina Larter, Yaguang Xi
      Pages 133-139

  5. High-Throughput Analysis of Small Non-Coding RNAs

    1. Front Matter

      Pages 141-141
      Download chapter PDF

    2. Microarray Analysis of Whole-Transcriptome RNAs Including Non-Coding RNAs

      • Quentin Thuillier, Isabelle Behm-Ansmant
      Pages 143-164

    3. Isolation, Extraction and Deep-Sequencing Analysis of Extracellular RNAs (exRNAs) from Human Plasma

      • Virginie Marchand, Adeline Galvanin, Yuri Motorin
      Pages 165-182
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Editors and Affiliations

  • Université de Lorraine, CNRS, IMoPA, Nancy, France

    Mathieu Rederstorff

Bibliographic Information

  • Book Title: Small Non-Coding RNAs

  • Book Subtitle: Methods and Protocols

  • Editors: Mathieu Rederstorff

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1386-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1385-6Published: 02 April 2021

  • Softcover ISBN: 978-1-0716-1388-7Published: 02 April 2022

  • eBook ISBN: 978-1-0716-1386-3Published: 01 April 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 270

  • Number of Illustrations: 18 b/w illustrations, 48 illustrations in colour

  • Topics: Genetics and Genomics

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