Abstract
The phytohormone (+)-7-iso-jasmonoyl-l-isoleucine (JA-Ile) causes protein–protein interactions (PPI) between F-box Protein CORONATINE INSENSITIVE 1 (COI1) and JASMONATE ZIM DOMAIN (JAZ) transcriptional repressor. A total of 13 JAZ subtypes are encoded in the genome of Arabidopsis thaliana; however, their genetic redundancy obfuscates the individual function of each JAZ. One approach to decipher this redundant signaling network is chemical genetics, using small molecules specific to individual JAZ subtype, for which a reliable and high-throughput screening system of the ligands for all combinations of COI1–JAZs would be indispensable. In this chapter, we describe a fluorescence anisotropy-based quantitative screening system for the ligands of COI1–JAZ co-receptors. Our method is applicable to agonists and antagonists of the COI1–JAZs.
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References
Santner A, Estelle M (2009) Recent advances and emerging trends in plant hormone signalling. Nature 459(7250):1071–1078
Chini A, Fonseca S, Fernandez G, Adie B, Chico JM, Lorenzo O, Garcia-Casado G, Lopez-Vidriero I, Lozano FM, Ponce MR, Micol JL, Solano R (2007) The JAZ family of repressors is the missing link in jasmonate signalling. Nature 448(7154):666–671
Thines B, Katsir L, Melotto M, Niu Y, Mandaokar A, Liu G, Nomura K, He SY, Howe GA, Browse J (2007) JAZ repressor proteins are targets of the SCF(COI1) complex during jasmonate signalling. Nature 448(7154):661–665
Yan J, Zhang C, Gu M, Bai Z, Zhang W, Qi T, Cheng Z, Peng W, Luo H, Nan F, Wang Z, Xie D (2009) The Arabidopsis CORONATINE INSENSITIVE1 protein is a jasmonate receptor. Plant Cell 21(8):2220–2236
Campos ML, Yoshida Y, Major IT, de Oliveira Ferreira D, Weraduwage SM, Froehlich JE, Johnson BF, Kramer DM, Jander G, Sharkey TD, Howe GA (2016) Rewiring of jasmonate and phytochrome B signalling uncouples plant growth-defense tradeoffs. Nat Commun 7:12570
Dejonghe W, Russinova E (2017) Plant chemical genetics: from phenotype-based screens to synthetic biology. Plant Physiol 174(1):5–20
Toth R, van der Hoorn RAL (2010) Emerging principles in plant chemical genetics. Trends Plant Sci 15(2):81–88
Takaoka Y, Iwahashi M, Chini A, Saito H, Ishimaru Y, Egoshi S, Kato N, Tanaka M, Bashir K, Seki M, Solano R, Ueda M (2018) A rationally designed JAZ subtype-selective agonist of jasmonate perception. Nat Commun 9(1):3654
Monte I, Hamberg M, Chini A, Gimenez-Ibanez S, Garcia-Casado G, Porzel A, Pazos F, Boter M, Solano R (2014) Rational design of a ligand-based antagonist of jasmonate perception. Nat Chem Biol 10(8):671–676
Matiolli CC, Melotto M (2018) A comprehensive Arabidopsis yeast two-hybrid library for protein-protein interaction studies: a resource to the plant research community. Mol Plant-Microbe Interact 31(9):899–902
Yan J, Yao R, Chen L, Li S, Gu M, Nan F, Xie D (2018) Dynamic perception of Jasmonates by the F-box protein COI1. Mol Plant 11(10):1237–1247
Burger M, Mashiguchi K, Lee HJ, Nakano M, Takemoto K, Seto Y, Yamaguchi S, Chory J (2019) Structural basis of Karrikin and non-natural Strigolactone perception in Physcomitrella patens. Cell Rep 26(4):855–865
Milroy LG, Grossmann TN, Hennig S, Brunsveld L, Ottmann C (2014) Modulators of protein-protein interactions. Chem Rev 114(9):4695–4748
Takaoka Y, Nagumo K, Azizah IN, Oura S, Iwahashi M, Kato N, Ueda M (2019) A comprehensive in vitro fluorescence anisotropy assay system for screening ligands of the jasmonate COI1-JAZ co-receptor in plants. J Biol Chem 294(13):5074–5081
Labbe CM, Laconde G, Kuenemann MA, Villoutreix BO, Sperandio O (2013) iPPI-DB: a manually curated and interactive database of small non-peptide inhibitors of protein-protein interactions. Drug Discov Today 18(19-20):958–968
Sheard LB, Tan X, Mao H, Withers J, Ben-Nissan G, Hinds TR, Kobayashi Y, Hsu FF, Sharon M, Browse J, He SY, Rizo J, Howe GA, Zheng N (2010) Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Nature 468(7322):400–405
Chini A, Gimenez-Ibanez S, Goossens A, Solano R (2016) Redundancy and specificity in jasmonate signalling. Curr Opin Plant Biol 33:147–156
Thireault C, Shyu C, Yoshida Y, St Aubin B, Campos ML, Howe GA (2015) Repression of jasmonate signaling by a non-TIFY JAZ protein in Arabidopsis. Plant J 82(4):669–679
Mizusawa K, Takaoka Y, Hamachi I (2012) Specific cell surface protein imaging by extended self-assembling fluorescent turn-on nanoprobes. J Am Chem Soc 134(32):13386–13395
Acknowledgments
This work was supported by a Grant-in-Aid for Scientific Research (no. 26282207, 17H06407, 17H00885 to MU, and No. 25708026, 18H02101 to Y.T.), JSPS A3 Foresight Program (to MU), JSPS Core-to-core Program ‘Asian Chemical Biology Initiative’ (to MU), and JST PREST (JPMJPR16Q4 to YT).
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Takaoka, Y., Hayashi, K., Suzuki, K., Azizah, I.N., Ueda, M. (2020). A Fluorescence Anisotropy-Based Comprehensive Method for the In Vitro Screening of COI1–JAZs Agonists and Antagonists. In: Champion, A., Laplaze, L. (eds) Jasmonate in Plant Biology. Methods in Molecular Biology, vol 2085. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0142-6_11
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DOI: https://doi.org/10.1007/978-1-0716-0142-6_11
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