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Jasmonate in Plant Biology

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2085)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Physiology and Development

    1. Front Matter

      Pages 1-1
    2. Multiple Roles of Jasmonates in Shaping Rhizotaxis: Emerging Integrators

      • Abdellah Lakehal, Alok Ranjan, Catherine Bellini
      Pages 3-22
  3. Biotic Interactions

    1. Front Matter

      Pages 41-41
    2. Role of Jasmonates in Beneficial Microbe–Root Interactions

      • Veronica Basso, Claire Veneault-Fourrey
      Pages 43-67
    3. Bioassays to Evaluate the Resistance of Whole Plants to the Herbivorous Insect Thrips

      • Merel Steenbergen, Colette Broekgaarden, Corné M. J. Pieterse, Saskia C. M. Van Wees
      Pages 93-108
    4. Role of Jasmonate in Modulation of Mycorrhizae-Induced Resistance Against Fungal Pathogens

      • Aswathy Nair, H. V. Thulasiram, Sujata Bhargava
      Pages 109-115
    5. Establishment of Actinorhizal Symbiosis in Response to Ethylene, Salicylic Acid, and Jasmonate

      • Mariama Ngom, Maimouna Cissoko, Krystelle Gray, Valérie Hocher, Laurent Laplaze, Mame Ourèye Sy et al.
      Pages 117-130
  4. Metabolism and Signaling

    1. Front Matter

      Pages 131-131
    2. Crystallization of a Complex Between MYC and Jas Motif

      • Feng Zhang, Sheng Yang He, Karsten Melcher
      Pages 133-144
    3. A Fluorescence Anisotropy-Based Comprehensive Method for the In Vitro Screening of COI1–JAZs Agonists and Antagonists

      • Yousuke Takaoka, Kengo Hayashi, Kaho Suzuki, Ika Nurul Azizah, Minoru Ueda
      Pages 145-160
    4. Use of Fluorescent Reporters to Analyse Dynamic and Spatial Responses to Mechanical Wounding

      • Antoine Larrieu, Trang Hieu Nguyen, Antony Champion
      Pages 161-168
    5. Detection of Fungal Jasmonates by Liquid Chromatography Paired with Mass Spectrometry

      • Yingyao Liu, Rajesh N. Patkar, Naweed I. Naqvi
      Pages 189-198
    6. CRISPR/Cas9-Mediated Gene Editing of the Jasmonate Biosynthesis OsAOC Gene in Rice

      • Trang Hieu Nguyen, Huong To Thi Mai, Daniel Moukouanga, Michel Lebrun, Stephane Bellafiore, Antony Champion
      Pages 199-209

About this book

This second edition volume expands on the previous edition with a look at the latest techniques used to study plant hormone jasmonate (JA). The chapters in this book and are organized into three parts: Parts One and Two discuss the role of JA in plant physiology and development, and in plant-biotic interactions. Part Three talks about methods used by researchers to study jasmonate metabolism and signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Jasmonate in Plant Biology: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field. 


Editors and Affiliations

  • UMR DIADE - IRD, Montpellier, France

    Antony Champion, Laurent Laplaze

Bibliographic Information

  • Book Title: Jasmonate in Plant Biology

  • Book Subtitle: Methods and Protocols

  • Editors: Antony Champion, Laurent Laplaze

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0142-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0141-9Published: 16 November 2019

  • Softcover ISBN: 978-1-0716-0144-0Published: 16 November 2020

  • eBook ISBN: 978-1-0716-0142-6Published: 16 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 228

  • Number of Illustrations: 31 b/w illustrations, 40 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access