Abstract
To mimic (human) cholestasis in vitro requires multiple triggers to establish a diseased phenotype. However, this is currently not simulated by existing in vitro models. Therefore, there is a high need for multicellular systems similar to the human physiology. In such an in vitro model, cell-cell interactions and intact bile canaliculi with functional bile flow should be present and preserved during long-term culture. Precision-cut liver slices represent an ex vivo tissue culture technique that replicates most of the multicellular characteristics of a whole liver in vivo. This chapter describes the preparation and culturing of (human) precision-cut liver slices. Furthermore, a protocol to use the precision-cut liver slices technique to predict drug-induced cholestatic liver injury is described.
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Acknowledgments
This work was financially supported by the grants of ZonMW (114000098, 114021010, 114025003, and 114022505) and EU Horizon2020 FETOPEN-01-2016-2017 (Project number: 190634130). Prof. Dr. E.M.J. Verpoorte for editing the manuscript.
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Karsten, R.E.H., Oosterhuis, D., van Wijk, L.A., Olinga, P. (2019). Ex Vivo Model in Cholestasis Research. In: Vinken, M. (eds) Experimental Cholestasis Research. Methods in Molecular Biology, vol 1981. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9420-5_23
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DOI: https://doi.org/10.1007/978-1-4939-9420-5_23
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