Abstract
Ribonucleotides are incorporated into genomes by DNA polymerases, they can be removed, and if not removed, they can have deleterious and beneficial consequences. Here, we describe an assay to quantify stable ribonucleotide incorporation by DNA polymerases in vitro, and an assay to probe for ribonucleotides in each of the two DNA strands of the yeast nuclear genome.
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Acknowledgments
We thank Mercedes Arana and Katarzyna Bebenek for thoughtful comments on the manuscript and Kunkel lab members for technical expertise and discussions. This work was supported by Project Z01 ES065070 to T.A.K. from the Division of Intramural Research of the National Institutes of Health (NIH), National Institute of Environmental Health Sciences (NIEHS).
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Clausen, A.R., Williams, J.S., Kunkel, T.A. (2015). Measuring Ribonucleotide Incorporation into DNA In Vitro and In Vivo. In: Vengrova, S., Dalgaard, J. (eds) DNA Replication. Methods in Molecular Biology, vol 1300. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2596-4_9
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DOI: https://doi.org/10.1007/978-1-4939-2596-4_9
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2595-7
Online ISBN: 978-1-4939-2596-4
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