Background

During yeast fermentation and fruit ripening short chain linear and branched alcohols are converted to their corresponding acetate esters by alcohol-O-acetyltransferase (AATase; EC 2.3.1.84; Figure 1). These volatile esters produce sweet and fruity fragrances: phenyl ethyl acetate smells of flowers, isoamyl acetate (isopentyl acetate) smells of bananas, and ethyl acetate smells of sweet pears. In plants, these and other esters function as attractors to pollinating species and as a defense mechanism, attracting predators to animals feeding on their leaves and fruit [1,2]. The function of microbial ester biosynthesis is not as well understood. AATase activity in Saccharomcyes cerevisiae is repressed by oxygen and unsaturated fatty acids [3-5] and it has been suggested that this activity functions as a means of CoA recycling with the co-production of organic acids [6,7], possibly as a response to stress conditions [8].

Figure 1
figure 1

Schematic of AATase pathway for ester biosynthesis.

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While there is uncertainty in the biological function of AATase activity in yeast, there are clear roles in metabolic engineering and industrial fermentations. The ester products have value as natural food additives, as aroma and flavor compounds in fermented beverages, and as industrial solvents [7,9]. The effects of AATase activity on aroma and flavor profiles in wine, beer, and sake fermentations are well understood [5,10-12]. The most well-studied AATases, Atf1 and Atf2 from S. cerevisiae, have been used to engineer whole cell E. coli catalysts for the conversion of ethanol and isoamyl alcohol to ethyl and isoamyl acetate [13-15] and for the biosynthesis of C4 to C11 volatile esters in E. coli [16]. An AATase from strawberry fruit (Fragaria species) has also been heterologously expressed in E. coli for the biosynthesis of butyl acetate and a range of butyrate esters [15,17]. Titers from these processes range from 0.04 – 0.23 g/L [13,15,17] to upwards of 17.5 g/L [16] and are, in part, limited by low AATase activity. In addition, the hydrophobic nature of these enzymes and varied intracellular localization of orthologs in their native hosts present complicating factors for heterologous expression in engineered hosts [8,18,19].

We have previously shown that Atf1 and −2 from S. cerevisiae localize to lipid droplets (LDs) via N- and C-terminal amphipathic helices [19]. The AATase ortholog from S. pastorianus also localizes to LDs by a similar mechanism, while AATases from non-Saccharomyces yeasts and fruit species, including Cucumis melo (melon), and Solanum lycopersicum (tomato) that do not have the conserved terminal helices from S. cerevisiae and do not localize to LDs. Early biochemical studies of Atf1 and −2 suggest that enzyme activity is membrane dependent. Purification in the presence of non-ionic detergents (e.g., hepthyl thioglucoside, octyl thioglucoside, and Triton-X100) resulted in measurable enzyme activity, while purification in the absence of such detergents resulted in inactive samples [6,20-22]. Due in part to this apparent membrane dependency as well as the hydrophobic nature of the AATase family, the standard activity assay has evolved to include Triton-X100 above the critical micelle concentration [23].

The apparent membrane dependency of Atf1 and −2 activity is interesting in the context of heterologous expression in E. coli or other microbial hosts for ester biosynthesis. Reported activities of homologously expressed Atf1 and −2 are moderate, ranging from 0.01 to 10 nmol min−1 per mg of protein of whole cell lysate [18,21,22,24], while the activity of orthologs from Pichia anomla and Klyuveromyces lactis are low (<1 nmol min−1 per mg of protein) [25]. Reported activities of strawberry AATases range from 8 – 75 nmol min−1 mg of enzyme [26,27]. The activities of Atf1 and −2 were measured in whole cell lysates that contain native LDs to which the enzymes can associate or in the presence of suitable membrane substitutes during purification. The successful metabolic engineering of E. coli to produce esters via an AATase pathway indicates that Atf1 and −2 maintain some activity in heterologous environments. In the absence of LDs Atf1 and −2 may associate with the plasma membrane, but the intracellular localization of Atf1 and −2 and other AATases heterologously expressed in E. coli and the effects of this localization on activity are not known.

In this work, a series of six AATases from Saccharomyces and non-Saccharomyces yeasts as well as tomato fruit (S. lycopersicum) were overexpressed in S. cerevisiae and E. coli and compared in terms of their intracellular localization, enzymatic activity, and expression level. The studies revealed that some AATases localize to LDs in S. cerevisiae and all studied AATases form enzymatically active aggregates in E. coli. Aggregate formation resulted in significantly reduced activities in comparison to activities measured in S. cerevisiae. The most active AATases, Atf1 from S. cerevisiae and Atf from S. lycopersicum, were used to demonstrate an expression strategy to partially recover the lost activity; reduced expression resulted in smaller aggregate size and higher specific activity.

Results

The strains, plasmids, and AATase genes used in this work are listed in Table 1. The genes include encoded AATases from Saccharomyces yeasts (Atf1-S.c, Atf2-S.c, and Atf1-S.p), and the non-Saccharomyces yeasts P. anomala (Atf-P.a), and K. lactis (Atf-K.l), as well as tomato fruit, S. lycopersicum (Atf-S.l). Preliminary activity screening from S. cerevisiae whole cell lysates with overexpressed AATases revealed that Atf1-S.c has the highest activity towards C2 to C5 alcohols with acetyl-CoA (Additional file 1: Table S1). As such, initial experiments focused on determining the intracellular localization and the enzymatic activity of Atf1-S.c towards ethyl acetate when overexpressed in S. cerevisiae and E. coli. Importantly, E. coli BL-21 without chloramphinicol acetyltransferase (CAT) activity was used, as CAT has been shown to exhibit AATase activity toward ethyl acetate synthesis (Additional file 1: Figure S1 and [16]).

Table 1 Strains, plasmids and primers used in this study
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In S. cerevisiae Atf1-S.c is known to localize to ER in early exponential phase and sort to LDs as cells progress in to stationary phase [19]. Atf1-S.c with a C-terminally fused GFP reporter co-localized with a fluorescently tagged LD marker, Erg6-DsRed, indicating LD localization in S. cerevisiae (Figure 2A, left). An overexpressed GFP control localized to the cytosol and fluorescent imaging did not indicate LD localization (Figure 2A, right). When overexpressed in E. coli, fluorescence microscopy revealed that GFP is cytosolically expressed and did not form visible aggregates or puncate structures (Figure 2B, right). In contrast, Atf1-S.c. formed aggregates when overexpressed in E. coli (Figure 2B, left). Nine percent of observed E. coli cells expressing Atf1-S.c. had one aggregate, 75% had two aggregates, and 16% had three aggregates, while no cells were observed with zero aggregates or four or more aggregates (Figure 2B, bottom). No E. coli cells expressing Atf1-S.c were observed to have an expression pattern similar to the GFP control, which showed fluorescence throughout the cell. Western blot analysis of protein expression in S. cerevisiae and E. coli showed that Atf1-S.c with a C-terminally fused GFP reporter expressed upwards of 100-fold more in E. coli than in S. cerevisiae (Figure 2C). Whole cell lysate activity assays of Atf1-S.c expressed in S. cerevisiae reached 58 ± 8 nmol min−1 mg−1 of protein. Despite the difference in expression levels, E. coli whole cell lysate activity assays where limited to 11.6 ± 0.1 nmol min−1 mg−1 of protein (Figure 2D).

Figure 2
figure 2

Expression, intracellular localization, and activity of Atf1 from S. cerevisiae (Atf1-S.c). A) Fluorescence microscopy images of S. cerevisiae co-expressing Atf1-GFP, GFP, and the LD marker Erg6-DsRed. Fluorescence from Erg6-DsRed is shown in red and fluorescence from Atf1-GFP is shown in green. Overlapping Erg6-DsRed and Atf1-GFP signals, indicating co-localization, are shown in yellow. GFP controls show cytosolic localization. Scale bar (1 μm) applies to A) and B). B) Fluorescent microscopy images of E. coli expressing Atf1-GFP and GFP. CellMask™ Orange plasma membrane staining is shown in red and fluorescence from Atf1-GFP and GFP is shown in green. Graphs below the fluorescence images indicate the number of aggregates observed in E. coli cells. A minimum of 100 cells were counted from three independent experiments. C) Western blot analysis of Atf1-GFP expression in S. cerevisiae (S.c) and E. coli. D) In vitro ethyl acetate production from whole cell lysates of Atf1-GFP expressed in S. cerevisiae and E. coli. Error bars represent standard deviation (n = 3).

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Similar intracellular localization results were observed with other studied AATases (Figure 3A,B). Atf2-S.c and Atf-S.p localized to LD in S. cerevisiae and formed aggregates when overexpressed in E. coli. Fluorescent microscopy imaging revealed that Atf-K.l with C-terminally fused GFP was soluble in S. cerevisiae, but formed aggregates in E. coli. Punctate structures were observed with Atf-P.a in S. cerevisiae and, similar to the other yeast AATases, formed aggregates in E. coli. Finally, Atf-S.l from tomato fruit appeared to localize homogenously throughout the cytosol of S. cerevisiae, but formed aggregates in E. coli. In each case, the majority of E. coli cells contained one or two aggregates of a given AATase with less than 8% of cells containing three aggregates (Figure 3B, bottom). All observed E. coli cells contained at least one aggregate and no cells were observed to contain more than three aggregates.

Figure 3
figure 3

Expression, intracellular localization, and activity of AATases. A) Fluorescence microscopy images of S. cerevisiae co-expressing AATase-GFP and the LD marker Erg6-DsRed. Expressed AATases include Atf2 from S. cerevisiae (Atf2-S.c), and AATases from S. pastorianus (Atf-S.p), K. lactis (Atf-K.l), P. anomala (Atf-P.a), and S. lycopersicum (Atf-S.l). Fluorescence from Erg6-DsRed is shown in red and fluorescence from AATase-GFP is shown in green. Overlapping Erg6-DsRed and GFP signals, indicating co-localization, are shown in yellow. Scale bar (1 μm) applies to A) and B). B) Fluorescent microscopy images of E. coli expressing AATases. CellMask™ Orange plasma membrane staining is shown in red and fluorescence from AATase-GFP is shown in green. Graphs below the fluorescence images indicate the number of aggregates observed in E. coli cells. A minimum of 100 cells were counted from three independent experiments. C) In vitro ethyl acetate production from whole cell lysates of AATase-GFP expressed in S. cerevisiae (S.c) and E. coli. Error bars represent standard deviation (n = 3). D) Western blot analysis of AATase-GFP expression in S. cerevisiae and E. coli.

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Whole cell lysate assays revealed that all AATases exhibited measurable activity when expressed in S. cerevisiae and E. coli (Figure 3C). Atf2-S.c activity in S. cerevisiae lysate was 1.2 ± 0.6 nmol min−1 mg−1, lower than the 3.1 ± 0.9 nmol min−1 mg−1 observed in E. coli lysate. Atf-S.p had activities of 0.3 ± 0.3 and 0.4 ± 0.1 nmol min−1 mg−1 in S. cerevisiae and E. coli, respectively. The activities of Atf-K.l and Atf-P.a reached 0.3 ± 0.2 and 0.4 ± 0.3 nmol min−1 mg−1 in S.cerevisiae, but only reached 0.02 ± 0.02 and 0.001 ± 0.001 nmol min−1 mg−1 in E. coli, respectively. Atf-S.l activity in E. coli lysate was limited at 0.5 ± 0.2 nmol min−1 mg−1; however, in S. cerevisiae lysate Atf-S.l exhibited activity of 21 ± 3 nmol min−1 mg−1, second only to the activity of Atf1-S.c. Western blot analysis revealed that in all cases AATase expression in E. coli was at least 100-fold greater than expression in S. cerevisiae (Figure 3D). In this context, normalization of activity to S. cerevisiae expression levels revealed that AATase activity in E. coli whole cell lysates for all AATases studied here is less than or equal to 0.08 nmol min−1 mg−1 of protein.

The ethyl acetate activities of Atf1-S.c and Atf-S.l when expressed in S. cerevisiae were significantly greater than the activities of all other studied orthologs. As such, subsequent experiments focused on these two enzymes. To determine if the AATase aggregates formed in E. coli were insoluble and active, we measured the activity of soluble and insoluble protein fractions after fractionation by centrifugation. Figure 4A shows that soluble lysate fractions maintained high activity for both Atf1-S.c and Atf-S.l in both hosts (Aft1-S.c: 45 ± 18 and 10 ± 3 nmol min−1 in S. cerevisiae and E. coli, respectively; Atf-S.l: 14 ± 4 and 2.2 ± 0.6 nmol min−1 in S. cerevisiae and E. coli, respectively). Atf1-S.c showed higher activity in the insoluble fractions of both S. cerevisiae and E. coli (50 ± 17 and 42 ± 6 nmol min−1, respectively). In contrast, the insoluble fractions containing overexpressed Atf-S.l showed measurable, but minimal activity (0.25 ± 0.12 and 1.3 ± 0.6 nmol min−1 mg−1 in S. cerevisiae and E. coli, respectively). The comparison of activity from S. cerevisiae and E. coli lysates is not complete without a similar comparison of expression levels in each host (Figure 4B). Western blots of S. cerevisiae lysates showed that Atf1-S.c separated equally between the soluble and insoluble fractions, but Atf-S.l separated strongly to the insoluble fraction. When expressed in E. coli, both Atf1-S.c and Atf-S.l were largely insoluble. A 100-fold dilution of the insoluble fraction of E. coli overexpressing Atf1-S.c and Atf-S.l resulted in western blot bands of near equal intensity to the soluble fractions with no dilution.

Figure 4
figure 4

Atf1-S.c and Atf-S.l activity in soluble and insoluble cell fractions. A) Comparison of AATase activity in soluble and insoluble fractions from S. cerevisiae (S.c) and E. coli, Atf1-S.c (left) and Atf-S.l (right). Error bars represent standard deviation (n = 3). B) Western blot analysis of soluble and insoluble fractions of Atf1-S.c and Atf-S.l from S. cerevisiae and E. coli expression.

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To further investigate the relationship between AATase overexpression in E. coli and enzymatic activity we modulated expression levels using a standard inducible T7 promoter system. Analysis of fluorescence microscopy images of E. coli expressing Atf1-S.c with C-terminal GFP tag revealed that intracellular aggregate size increased from 0.11 ± 0.05 μm2 when induced with 1 μM IPTG to 0.19 ± 0.08 μm2 with 10 μM IPTG (Figure 5A and Additional file 1: Figure S2). Aggregate size further increased to 0.44 ± 0.20 μm2 with 100 μM IPTG. Coincident with increased induction and aggregate size were Atf1-S.c. expression levels (Figure 5B and Additional file 1: Figure S3). Induction with 10 and 100 μM IPTG resulted in 13.5 ± 5.1 and 28.5 ± 5.1 fold increases in protein expression over levels observed with 1 μM IPTG induction. Similar aggregate size and protein expression levels were observed with Atf-S.l. Under low induction conditions, aggregates of Atf-S.l with a C-terminal GFP tag were found to be 0.11 ± 0.04 μm2. Aggregate size increased to 0.20 ± 0.07 and 0.41 ± 0.15 μm2 when induced with 10 and 100 μM IPTG, respectively. Quantification of western blots showed that protein expression increased by 7.8 ± 1.6 and 16.5 ± 6.6 fold with increased induction levels of 10 and 100 μM IPTG, respectively (Figure 5C).

Figure 5
figure 5

Reduced AATase expression decreases aggregates size in E. coli fermentations. A) Fluorescence microscopy images and aggregate size measurement of Atf1-S.c and Atf-S.l in E. coli. Scale bar is 1 μm. Error bars represent standard deviation (n = 100), * p < 0.05. B, C) Relative expression levels of Atf1-S.c (B) and Atf-S.l (C) in E. coli as judged by western blot analysis. Expression levels are normalized to the intensity of anti-GFP signal from E. coli induced with 1 μM IPTG. D, E) Normalized AATase activity from Atf1-S.c (D) and Atf-S.l (E) during 24 hour E. coli fermentations at 30°C. Activities are normalized to the lowest expression level, IPTG induction of 1 μM. Error bars represent standard deviation (n = 3).

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The result of reduced expression level and aggregate size was an increase in specific AATase activity during fermentation. Figures 5D shows Atf1-S.c activity in terms of ethyl acetate produced during a 24-hour fermentation normalized to the activity at the lowest protein expression level (i.e., induction with 1 μM IPTG). Increased expression of Atf1-S.c reduced specific activity by 72.6 ± 13.1% and 84.2 ± 2.7% with 10 and 100 μM IPTG, respectively (Figure 5D). Despite the considerable decrease in specific activity, high expression levels of Atf1-S.c resulted in increased ethyl acetate production under fermentation. For Atf1-S.c, the amount of ethyl acetate produced in 24-hours of fermentation increased from 1.35 ± 0.10 to 4.78 ± 0.28 and 6.74 ± 0.24 mg/L with induction from 1 to 10 and 100 μM IPTG, respectively, while the amounts of ethanol produced under the same conditions were 0.87 ± 0.07, 0.89 ± 0.04, and 0.94 ± 0.01 g/L, respectively (Additional file 1: Figure S4). It is important to note that culture density measured by absorbance at 600 nm, A600, was unchanged with varied induction levels when expressing Atf1-S.c and a small but statistically significant reduction in A600 was observed between the lowest and highest induction levels when expressing Atf-S.l (A600 of 3.64 ± 0.44 and 2.48 ± 0.36, respectively; Additional file 1: Figure S5). As shown in Figure 5B, Atf1-S.c expression increased by upwards of 28-fold under the same conditions, considerably more than the fold increase in ethyl acetate production, indicating that increasing enzyme expression did not result in a proportional increase in ester synthesis. Importantly, the increase in ethyl acetate production with increased expression of Atf1-S.c suggested that AATase activity is rate limiting for the conversion of ethanol to ethyl acetate and that the observed loss in activity is due to the enzyme and not an upstream pathway bottleneck. The low conversions of ethanol to ethyl acetate are also supportive of Atf1-S.c activity as rate limiting.

During 24 hours of E. coli fermentation with low expression of Atf-S.l, 0.30 ± 0.09 mg/L of ethyl acetate and 0.95 ± 0.20 g/L of ethanol were produced; there was no statistically significant different in the production of either compound with increased induction (Additional file 1: Figure S4). The low levels of ethyl acetate production in comparison to fermentation with Atf1-S.c expressing E. coli were expected due to lower activity of Atf-S.c ; however, varying expression levels of Atf-S.l produced a similar trend in normalized specific activity to that observed with Atf1-S.c. At higher expression levels specific activity was reduced by 85.6 ± 2% and 90.3 ± 4% with inductions of 10 and 100 μM IPTG, respectively (Figure 5E). To confirm the effects of varying expression on activity, whole cell lysate assays and corresponding western blots were performed. For both Atf1-S.c and Atf-S.l the relative protein levels in whole cell lysates increased with increased induction (Figure 6A,B). Similar to the fermentation studies, the increased protein levels did not result in a proportional increase in ethyl acetate synthesis and normalized specific activity decreased significantly with induction levels of 10 and 100 μM IPTG. For Atf1-S.c the highest protein levels resulted in less of a reduction in activity then with intermediate protein levels (residual activities of 12.4 ± 2.0% and 43.3 ± 6.9% for 10 and 100 μM IPTG, respectively). For Atf-S.l increased protein levels due to induction with 10 and 100 μM IPTG measured specific activities were less than 5% of the specific activity from 1 μM IPTG induction.

Figure 6
figure 6

Reduced AATase expression increases specific activity in whole cell lysate assays. A,B) Atf1-S.c (A) and Atf-S.l (B) activity in E. coli whole cell lysates normalized to the expression level from induction with 1 μM IPTG. Error bars represent standard deviation (n = 3). C,D) Western blot analysis of Atf1-S.c (C) and Atf-S.l (D) expression levels in E. coli with inductions of 1, 10, and 100 μM IPTG.

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Discussion

A major challenge in engineering metabolic pathways, including ester biosynthesis, is pathway optimization including the alleviation of kinetics bottlenecks and balancing the kinetic capacity of pathway steps. One approach to maximizing flux is pathway engineering to alter the expression levels of each step [28,29]. Enzyme engineering to improve the kinetics of key steps is also critical [30]. The difficulty of these approaches is compounded when native intracellular localization, activity, and expression of key enzymes are effected by heterologous expression. Flux analysis and pathway modeling are also negatively affected because experimentally determined kinetic parameters used in modeling do not accurately translate to those exhibited in vivo. The AATases studied here are prime examples of these problems as heterologous expression in E. coli resulted in losses in specific activity and in some cases a loss of intracellular localization.

Homologous expression of Atf1-S.c in S. cerevisiae revealed high activity in whole cell lysates and native localization to LDs. Heterologous expression in E. coli was approximately 100-fold higher than in S. cerevisiae, but whole cell lysate assays revealed significantly reduced specific activity and in vivo fluorescence imaging revealed a loss of membrane localization (Figure 2). These results were consistent with Atf2-S.c from S. cerevisiae and Atf-S.p from S. pastorianus. Enzymes localized to LD in S. cerevisiae, but membrane localization was not observed in E. coli (Figure 2A,B). Despite high expression in E. coli, specific activities of Atf2-S.c and Atf-S.p in E. coli cell lysates were significantly less than observed in S. cerevisiae lysates. AATases from K. lactis, S. lycopersicum, and P. anomala did not localized to LD or other membranes when expressed in S. cerevisiae and did not localized to membranes in E. coli. Again, expression in E. coli was high and specific activities measured in whole cell lysates were significantly reduced in comparison with activities measured in S. cerevisiae lysates. Previous work investigating the localization of AATases in S. cerevisiae has shown that minimal modifications to the C-terminus did not affect the membrane localization of Atf1-S.c, Atf2-S.c, or Atf-S.p and that the C-terminal GRF reporter did not prevent localization [19]. Conserved N- and C-terminal amphipathic helices were identified as necessary for AATase membrane localization, motifs that are lacking in Atf-K.l, −P.a, and -S.l. In this context, it is unlikely that the C-terminal GFP reporter alters membrane localization; however, this remains as a possible explanation for the lack of AATase localization in S. cerevisiae.

A contributing factor to the loss of activity was AATase aggregation in E. coli. Fluorescence imaging of E. coli cells revealed that in all cases expressed AATase formed one or more cytosolic aggregates (Figure 2B, 3B). Importantly, the aggregates were functional. We expected that loss of tertiary structure in inclusion bodies would destroy protein function, but in all cases C-terminally fused GFP maintained fluorescence and whole cell lysates exhibited AATase activity suggesting that AATases expressed in E. coli were not misfolded [31]. Moreover, analysis of fractionated E. coli lysates containing Atf1-S.c or Atf-S.1 demonstrated measurable activity in both the soluble and insoluble fractions (Figure 4). Common to the AATase family are short stretches of hydrophobic amino acids that are a possible source of protein aggregation [8,18]. Specific to AATases from S. cerevisiae and S. pastorianus, the N- and C-terminal amphipathic helices that function as ER and LD membrane anchors may also be a source of aggregation [19]. Regardless of mechanism(s), the results of heterologous expression in E. coli were clear, the formation of AATase active aggregates. The loss of activity was most likely due to a combination of diffusion limitations and blocked active sites. The results demonstrating increased specific activity at reduced expression levels supports these conclusions (Figures 5 and 6). Reduced expression of Atf1-S.c and Atf-S.l in E. coli produced smaller aggregates and increased measures of specific activity both in whole cell lysate assays and under fermentation conditions. Reduction in aggregate size increases the ratio of surface to interior proteins thus minimizing blocked active sites. It is also possible that activity in aggregates was due to the disruption of active protein conformation, an effect that is also likely decreased with aggregate size. An alternative explanation is that AATase solubility was increased at lower induction levels, an effect that has previously been demonstrated with single chain antibodies expressed in an E. coli host [32]. An additional means of decreasing expression and increasing solubility is culturing at lower temperatures; however, fluorescence microscopy revealed that expression in E. coli at 20 and 30°C produced aggregates (Additional file 1: Figure S6).

Biochemical studies of Atf1 from S. cerevisiae (Atf1-S.c) have suggested that activity is membrane-dependent [6,20-22]. The studies presented here confirm that Atf1-S.c, Atf2-S.c and Atf-S.p localize to LDs in S. cerevisiae, but do not localize to membranes in E. coli. The loss of membrane localization did not eliminate activity towards ethyl acetate suggesting that these AATases are not strictly membrane dependent with respect to activity (Figures 2 and 3). Published purification protocols for Atf1-S.c use surfactants or non-ionic detergents and in their absence activity is significantly reduced. In our hands, nickel-affinity chromatography purification of Atf1-S.c resulted in AATase active samples (Additional file 1: Figure S7), thus supporting the claim that membrane localization is not necessary for enzymatic activity. This lack of strict membrane dependency provides an explanation as to why metabolic engineering of E. coli to synthesize short chain volatile esters has been successful, and our analysis of AATase expression and activity in S. cerevisiae and E. coli hosts provides important information for future metabolic engineering of ester biosynthesis.

Conclusions

Ester biosynthesis is a promising new target for metabolic engineering. The market price of ethyl and butyl acetates are upwards of $1500 per tonne (http://www.icis.com/), and the total market value for flavor and fragrance compounds is greater than $16 billions (http://www.ialconsultants.com/). The high volatility of shorter chain esters such as ethyl acetate is also attractive from a separation perspective, as high volatility facilitates separation from fermentation broths [33]. The results presented here demonstrate that microbial host selection is critical to ethyl acetate biosynthesis through AATase activity. Heterologous AATase expression in E. coli resulted in significantly decreased specific activity in comparison to activity measure in S. cerevisiae. All studied AATases formed aggregates when expressed in E. coli and any membrane localization in S. cerevisiae was lost in E. coli. One solution to minimizing the loss of activity is reduced expression levels in E. coli, which resulted in smaller aggregate size and increased specific activity in comparison to high overexpression. The effects of host selection on AATase expression and activity described here are interesting in that they provide evidence that the AATases studied here are not strictly membrane dependent with respect to activity and are important when considering metabolic engineering strategies for ester biosynthesis.

Methods

Strains, plasmids, and culture conditions

Strains and plasmids used in this work are shown in Table 1. E. coli strains were grown in LB medium containing 30 μg/mL kanamycin. S.cerevisiae strains were prepared as previously described [19], and were grown in synthetic minimal (SD) medium containing 0.67% yeast nitrogen base (Becton-Dickinson), amino acid supplements (Sunrise), and 2% glucose. Expression in E. coli was induced by adding IPTG at OD600 of 0.4.

Preparation of whole cell lysate

Cells were harvested by centrifugation at 3,500 rpm for 5 min at 4°C and washed twice with 100 mM potassium phosphate buffer (pH 7.4) containing 2 mM magnesium chloride. Equal volumes of wet cell pellets and 425–600 μm acid-washed glass beads (Sigma-Aldrich, G8772) were added to a 15 mL tube and resuspended in 1 mL ice-cold lysis buffer (100 mM potassium phosphate buffer, 2 mM magnesium chloride, 2 mM DTT, and protease inhibitor). The cells were disrupted by vortexing 10 times for 30 s. After each vortexing the suspension was kept on ice for 30 s. The beads were removed by centrifugation at 500 g for 5 min at 4°C, and the supernatant was decanted to a cold 2 mL tube. The protein concentrations of whole cell lysates were determined by Thermo Scientific Pierce 660 nm Protein Assay.

Enzyme activity assays

AATase activity was measured using ethanol and acetyl-CoA as substrates. A reaction mixture contains 100 mM potassium phosphate (pH 7.4), 500 mM ethanol and 0.5 mM acetyl-CoA and 100 μg lysate was used. After incubation at 30°C for 0.5 hours, the reaction was stopped by the addition 60 μmol H2SO4. 100 μg of 1-pentanol was added as an internal standard and 1 g NaCl was added to reduce the solubility of ethyl acetate. The concentration of ethyl acetate produced was measured by headspace gas chromatography. To determine the activity of soluble and insoluble cell fractions, whole cell lysates were centrifuged at 15,000 rpm for 20 min at 4°C. The supernatant was isolated and taken as the soluble fraction. The pellet was washed twice with lysis buffer before re-suspending in lysis buffer. The activity of the re-suspended pellet and soluble fraction were measured as described above.

Fermentations

E. coli strains were grown in Terrific Broth containing 2% glucose and 30 μg/mL kanamycin with IPTG induction at OD600 of 0.4. Cells were cultured anaerobically in 125-mL screwed cap flask at 30°C for 24 hours on a rotary shaker. The shake flask headspace was purged with nitrogen prior to incubation.

Ethyl acetate detection

Produced ethyl acetate was quantified by a headspace gas chromatography with a flame ionization detector (Agilent Technologies 7890A GC with CTC-PAL headspace mode injector). The separation of volatile compounds was carried out by Rtx®-1 column (30 m, 0.32 mmID, 5 μm film thickness; RESTEK) with helium as carrier gas. GC oven temperature was initially at 75°C for 7 min and increased with a gradient of 30°C/min until 175°C, followed by a gradient of 50°C/min until 275°C. The injector and detector were held at 275°C. 1 mL headspace gas was injected to the GC and 1-pentanol was used as internal standard.

Fluorescent microscopy and image analysis

Cells were observed as described in [19]. Briefly, an Olympus BX51 microscope (UPlanFL 100X 1.30 oil-immersion objective lense, mercury lamp) with Q-Imaging Retiga Exi CCD camera was used to capture images. CellSens Dimension 1.7 software (Olympus) was used to process images. Image J software was used to measure protein aggregate size. Quantitative values of aggregates size and number are from a minimum of 100 cells. Statistical analysis of aggregate size was accomplished by one-way analysis of variance. A p value <0.05 was applied for statistical significance.

Western blot analysis and quantification of protein expression

Western blots were performed using standard procedure. Protein extracts were loaded on Any kD™ Mini-PROTEAN® TGX™ Gel (Bio-Rad) and run at 150 V for 1 hour. Samples were electrophoretically transferred to a PVDF membrane at 25 V overnight. Membranes were blocked with 5% non-fat milk in TBST buffer for 1 hour at room temperature and incubated with GFP Rabbit Serum Polyclonal Antibody (Life Technologies) diluted to 1: 20000 in TBST buffer with 1% non-fat milk. Goat Anti-Rabbit IgG-HRP (Life Technologies) diluted to 1: 10000 was added as secondary antibody and incubated at room temperature for 0.5 hours. After washing with TBST, HRP substrate (Bio-Rad) was used for signal detection. Image-J software was used to quantify band intensity.