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Natamycin release from alginate active films to liquid and semi-solid media

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Abstract

Alginate films containing natamycin as the antimicrobial agent were developed and its release from the films to liquid and semi-solid media with different water activities was evaluated. In samples immersed in liquid media, the release rate of natamycin increased with an increase in the water activity of the solutions. Effective diffusivities were estimated by fitting Fick’s second law to the data, obtaining values ranging from 0.52 × 10–12 to 5.30 × 10–12 cm2/s. The amount of natamycin that migrated to the semi-solid media indicated incomplete release, with 85% of the initial mass released in gels and only 33% released in cheese samples. This highlights the importance of evaluating the release behavior in real systems. The films were applied to slices of semi-hard cheese, previously inoculated with Aspergillus niger and Penicillium roqueforti. Colony counts on the surface of cheese samples sandwiched between active films were one log cycle lower than that in uncovered slices. Cheese samples covered with control films (without natamycin) also yielded fewer colonies but were not as effective.

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References

  • Berti S, Resa CPO, Basanta F, Gerschenson LN, Jagus RJ (2019) Edible coatings on Gouda cheese as a barrier against external contamination during ripening. Food Biosci 31:100447

    Article  CAS  Google Scholar 

  • Bierhalz ACK, da Silva MA, de Sousa HJC, Braga MEM, Kieckbusch TG (2013) Influence of natamycin loading methods on the physical characteristics of alginate active films. J Supercrit Fluid 76:74–82

    Article  CAS  Google Scholar 

  • Bierhalz ACK, da Silva MA, Braga MEM, Sousa HJC, Kieckbusch TG (2014) Effect of calcium and/or barium crosslinking on the physical and antimicrobial properties of natamycin-loaded alginate films. LWT Food Sci Technol 57:494–501

    Article  CAS  Google Scholar 

  • Bonilla J, Sobral PJ (2019) Gelatin-chitosan edible film activated with Boldo extract for improving microbiological and antioxidant stability of sliced Prato cheese. Int J Food Sci Technol 54:1617–1624

    Article  CAS  Google Scholar 

  • Campos CA, Gerschenson LN, Flores SK (2011) Development of edible films and coatings with antimicrobial activity. Food Bioprocess Technol 4:849–875

    Article  CAS  Google Scholar 

  • Costa MJ, Marques AM, Pastrana LM, Teixeira JA, Sillankorva SM, Cerqueira MA (2018) Physicochemical properties of alginate-based films: effect of ionic crosslinking and mannuronic and guluronic acid ratio. Food Hydrocoll 81:442–448

    Article  CAS  Google Scholar 

  • Crank J (1979) The mathematics of diffusion. Oxford University Press, London

    Google Scholar 

  • Cruz JM, Silva AS, García RS, Franz R, Losada PP (2008) Studies of mass transport of model chemicals from packaging into and within cheeses. J Food Eng 87:107–115

    Article  CAS  Google Scholar 

  • da Silva MA, Bierhalz ACK, Kieckbusch TG (2009) Alginate and pectin composite films crosslinked with Ca2+ ions: effect of the plasticizer concentration. Carbohydr Polym 77:736–742

    Article  Google Scholar 

  • da Silva MA, Bierhalz ACK, Kieckbusch TG (2012) Modelling natamycin release from alginate/chitosan active films. Int J Food Sci Technol 47:740–746

    Article  Google Scholar 

  • de Oliveira TM, Soares NFF, Pereira RM, Fraga KF (2007) Development and evaluation of antimicrobial natamycin incorporated film in gorgonzola cheese conservation. Packag Technol Sci 20:147–153

    Article  Google Scholar 

  • del Sol González-Forte L, Amalvy JI, Bertola N (2019) Corn starch-based coating enriched with natamycin as an active compound to control mold contamination on semi-hard cheese during ripening. Heliyon 5:e01957

    Article  Google Scholar 

  • Fajardo P, Martins JT, Fuciños C, Pastrana L, Teixeira JA, Vicente AA (2010) Evaluation of a chitosan-based edible film as carrier of natamycin to improve the storability of Saloio cheese. J Food Eng 101:349–356

    Article  CAS  Google Scholar 

  • Han JH (ed) (2014) Innovations in food packaging, 2nd edn. Academic Press, London

    Google Scholar 

  • He C, Zhang Z, Li B, Xu Y, Tian S (2019) Effect of natamycin on Botrytis cinerea and Penicillium expansum—postharvest pathogens of grape berries and jujube fruit. Postharvest Biol Tec 151:134–141

    Article  CAS  Google Scholar 

  • Küçük GS, Çelik ÖF, Mazi BG, Türe H (2020) Evaluation of alginate and zein films as a carrier of natamycin to increase the shelf life of kashar cheese. Packag Technol Sci 33:39–48

    Article  Google Scholar 

  • Kure CF, Skaar I (2019) The fungal problem in cheese industry. Curr Opin Food Sci 29:14–19

    Article  Google Scholar 

  • Lin L, Gu Y, Cui H (2019) Moringa oil/chitosan nanoparticles embedded gelatin nanofibers for food packaging against Listeria monocytogenes and Staphylococcus aureus on cheese. Food Packag Shelf 19:86–93

    Article  Google Scholar 

  • Lin M, Fang S, Zhao X, Liang X, Wu D (2020) Natamycin-loaded zein nanoparticles stabilized by carboxymethyl chitosan: evaluation of colloidal/chemical performance and application in postharvest treatments. Food Hydrocol 106:105871

    Article  CAS  Google Scholar 

  • Luz C, Torrijos R, Quiles JM, Mañes J, Meca G (2019) Shelf life extension of mozzarella cheese contaminated with Penicillium spp. using the antifungal compound ɛ-polylysine. Food Sci Technol Int 25:295–302

    Article  CAS  Google Scholar 

  • Mahcene Z, Khelil A, Hasni S, Bozkurt F, Goudjil MB, Tornuk F (2020) Home-made cheese preservation using sodium alginate based on edible film incorporating essential oils. J Food Sci Technol 58(6):2406–2419

    Article  Google Scholar 

  • Nešić A, Cabrera-Barjas G, Dimitrijević-Branković S, Davidović S, Radovanović N, Delattre C (2020) Prospect of polysaccharide-based materials as advanced food packaging. Molecules 25:135

    Article  Google Scholar 

  • Nottagh S, Hesari J, Peighambardoust SH, Rezaei-Mokarram R, Jafarizadeh-Malmiri H (2020) Effectiveness of edible coating based on chitosan and Natamycin on biological, physico-chemical and organoleptic attributes of Iranian ultra-filtrated cheese. Biologia 75:605–611

    Article  CAS  Google Scholar 

  • Petersson M, Hagström J, Nilsson K, Stading M (2007) Kinetics of release from kafirin films. Food Hydrocoll 21:1256–1264

    Article  CAS  Google Scholar 

  • Pires ACS, Soares NFF, de Andrade NJ, da Silva LHM, Camilloto GP, Bernardes PC (2008) Development and evaluation of active packaging for sliced mozzarella preservation. Packag Technol Sci 21:375–383

    Article  Google Scholar 

  • Resa CPO, Gerschenson LN, Jagus RJ (2014) Natamycin and nisin supported on starch edible films for controlling mixed culture growth on model systems and Port Salut cheese. Food Control 44:146–151

    Article  Google Scholar 

  • Romero V, Borneo R, Passalacqua N, Aguirre A (2016) Biodegradable films obtained from triticale (x Triticosecale Wittmack) flour activated with natamycin for cheese packaging. Food Packag Shelf 10:54–59

    Article  Google Scholar 

  • Santonicola S, García Ibarra V, Sendón R, Mercogliano R, Rodríguez-Bernaldo de Quirós A (2017) Antimicrobial films based on chitosan and methylcellulose containing natamycin for active packaging applications. Coatings 7:177

    Article  Google Scholar 

  • Saravani M, Ehsani A, Aliakbarlu J, Ghasempour Z (2019) Gouda cheese spoilage prevention: biodegradable coating induced by Bunium persicum essential oil and lactoperoxidase system. Food Sci Nutr 7:959–968

    Article  CAS  Google Scholar 

  • Zactiti EM, Kieckbusch TG (2009) Release of potassium sorbate from active films of sodium alginate crosslinked with calcium chloride. Packag Technol Sci 22:349–358

    Article  CAS  Google Scholar 

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Acknowledgements

The authors acknowledge the CNPq (Grant Number 473972/2011-5 and 140303/2012-0) for financial support.

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Authors and Affiliations

  1. Department of Engineering, Federal University of Santa Catarina-Campus Blumenau, Rua João Pessoa 2740, Blumenau, SC, 89036-256, Brazil

    Andréa C. K. Bierhalz

  2. Center of Agricultural Sciences, Federal University of São Carlos, Rodovia Anhanguera, km 174, Araras, SP, 13600-970, Brazil

    Mariana A. da Silva

  3. School of Chemical Engineering, University of Campinas, Av. Albert Einstein 500, Campinas, SP, 13083-852, Brazil

    Theo G. Kieckbusch

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  1. Andréa C. K. Bierhalz
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  2. Mariana A. da Silva
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  3. Theo G. Kieckbusch
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Correspondence to Andréa C. K. Bierhalz.

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Bierhalz, A.C.K., da Silva, M.A. & Kieckbusch, T. . Natamycin release from alginate active films to liquid and semi-solid media. Braz. J. Chem. Eng. 39, 455–462 (2022). https://doi.org/10.1007/s43153-021-00139-w

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