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Detection of the international lineage ST71 of methicillin-resistant Staphylococcus pseudintermedius in two cities in Rio de Janeiro State

  • Veterinary Microbiology - Short Communication
  • Published:
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Abstract

Staphylococcus pseudintermedius is the main coagulase-positive staphylococci associated with canine skin/soft tissue infections (SSTI), otitis externa, and surgical site infections. The international spread of an epidemic and multiresistant lineage of methicillin-resistant Staphylococcus pseudintermedius (MRSP), the so-called European clone—displaying sequence type (ST) 71—requires attention. The first isolation of an MRSP ST71 isolate in South America was reported in Rio de Janeiro city, in 2010; however, a limited number of canine isolates were analyzed. Thus, to have a better panel of the MRSP spread in this city, we were stimulated to continue this study and search for the presence of MRSP in 282 colonized or infected dogs in the city of Rio de Janeiro. Among the MRSP isolates collected (N = 17; 6.1%), the pulsed-field gel electrophoresis (PFGE) patterns were similar to those of European clone. All 17 isolates were classified as ST71 by multilocus sequence typing (MLST). In order to assess whether isolates of MRSP ST71 may have also spread to the Rio de Janeiro state countryside, we collected samples from 124 infected dogs in the city of Campos dos Goytacazes (232 km away from Rio de Janeiro city). Our data showed the presence of ST71 lineage in one isolate among three MRSP detected. S. pseudintermedius was isolated from 40.6% of the clinical samples (N = 165/406). A relatively high incidence of methicillin resistance, detected by a PCR-based method, was found in 12.1% of the S. pseudintermedius recovered from animals (N = 20/165). The resistance profile of these isolates was similar to that described for the international ST71 strains whose genomes are publicly available in the GenBank. The prospect of ST71 isolates being resistant to virtually all antimicrobials used in veterinary medicine is alarming and should be considered a central issue considering that MRSP ST71 spreads over large geographic distances and its transmission from animals to humans.

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Funding

This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), grant # 307672/2019–0, and by Fundação de Amparo à Pesquisa do Rio de Janeiro (FAPERJ), grants E-26/010.101098/2018, E-26/010.001280/2016, E-26/010.002435/2019, and E-26/200.952/2021. This study was also financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES) Finance Code 001.

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Author notes

  1. Bruno Penna and Marcella B. Silva contributed equally to this work.

Authors and Affiliations

  1. Laboratório de Cocos Gram Positivos, Instituto Biomédico, Universidade Federal Fluminense, Niteroi, Brazil

    Bruno Penna, Ana M. N. Botelho & Renata F. Rabello

  2. Laboratório de Biologia Molecular de Bactérias Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal Do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil

    Bruno Penna, Marcella B. Silva, Fabienne A. Ferreira, Mariana S. Ramundo, Maria C. Silva-Carvalho & Agnes M. S. Figueiredo

  3. Laboratório de Sanidade Animal - Setor Doenças Infectocontagiosas, Universidade Estadual Do Norte Fluminense Darcy Ribeiro (UENF), Macae, Brazil

    Marcella B. Silva & Olney Vieira-da-Motta

  4. Laboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Imunologia E Parasitologia, Universidade Federal de Santa Catarina (UFSC), Florianopolis, Brazil

    Fabienne A. Ferreira

  5. Departamento de Moléstias Infecciosas E Parasitárias, Faculdade de Medicina, Instituto de Medicina Tropical, Universidade de São Paulo (IMT-FMUSP), Sao Paulo, Brazil

    Mariana S. Ramundo

Authors
  1. Bruno Penna
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  2. Marcella B. Silva
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  6. Maria C. Silva-Carvalho
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  9. Agnes M. S. Figueiredo
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Contributions

BP collected animal samples, carried out strain isolation and molecular characterization, and wrote the manuscript. MBS collected animal samples, carried out strain isolation and identification, and wrote the draft of the manuscript. AMNB performed the genomic database construction and genomics analysis and contributed to the final version of the manuscript. FAF and MSR performed molecular typing by MLST. MCSC and RFR performed molecular characterization by PFGE. AMSF, BF, and OVM were responsible for the study design and wrote the final version of the manuscript. All authors approved the final version of the manuscript.

Corresponding author

Correspondence to Bruno Penna.

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Ethics approval

This study was approved by the animal ethics committees from Universidade Federal Fluminense (#218/2010) and Universidade Estadual do Norte Fluminense (#145/2011).

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The authors declare no competing interests.

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Responsible Editor: Luis Augusto Nero

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Penna, B., Silva, M.B., Botelho, A.M.N. et al. Detection of the international lineage ST71 of methicillin-resistant Staphylococcus pseudintermedius in two cities in Rio de Janeiro State. Braz J Microbiol 53, 2335–2341 (2022). https://doi.org/10.1007/s42770-022-00852-9

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