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Tibouchina granulosa (Vell.) Cogn (Melastomataceae) as source of endophytic fungi: isolation, identification, and antiprotozoal activity of metabolites from Phyllosticta capitalensis

  • Biotechnology and Industrial Microbiology - Research Paper
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Abstract

Endophytes are microorganisms that form symbiotic relationships with their own host. Included in this group are the species Phyllosticta capitalensis, a group of fungi that include saprobes that produce bioactive metabolites. The present study aimed to identify the cultivable endophytic fungal microbiota present in healthy leaves of Tibouchina granulosa (Desr.) Cogn. (Melastomataceae) and investigate secondary metabolites produced by a strain of P. capitalensis and their effects against both Leishmania species and Trypanossoma cruzi. Identification of the strains was accomplished through multilocus sequencing analysis (MLSA), followed by phylogenetic analysis. The frequency of colonization was 73.66% and identified fungi belonged to the genus Diaporthe, Colletotrichum, Phyllosticta, Xylaria, Hypoxylon, Fusarium, Nigrospora, and Cercospora. A total of 18 compounds were identified by high-resolution mass spectrum analysis (UHPLC-HRMS), including fatty acids based on linoleic acid and derivatives, from P. capitalensis. Crude extracts had activity against Leishmania amazonensis, L. infantum, and Trypanosoma cruzi, with inhibitory concentration (IC50) values of 17.2 μg/mL, 82.0 μg/mL, and 50.13 μg/mL, respectively. This is the first report of the production of these compounds by the endophytic P. capitalensis isolated from T. granulosa.

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Funding

The authors are grateful to CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for scholarships (Finance code 001) and to CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) (307603/2017-2) and the SETI/UGF (TC n.65/2018) for financial support.

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Authors and Affiliations

  1. Laboratório de Biologia, Universidade Tecnológica Federal do Paraná, Apucarana, Paraná, CEP 86812-460, Brazil

    Halison Correa Golias

  2. Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, Paraná, CEP: 87020-900, Brazil

    Halison Correa Golias, Julio Cesar Polonio, Marcos Alessandro dos Santos Ribeiro, Andressa Domingos Polli, Angela Aparecida da Silva, Aline Maria Bulla & João Alencar Pamphile

  3. Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos, Universidade Estadual de Maringá, Maringá, Paraná, CEP: 87020-900, Brazil

    Hélito Volpato & Celso Vataru Nakamura

  4. Universidade Federal do Paraná, Jandaia do Sul, Paraná, CEP: 86900-000, Brazil

    Eduardo Cesar Meurer

  5. Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz – ESALQ/USP, Piracicaba, São Paulo, CEP: 13418-260, Brazil

    João Lúcio Azevedo

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  1. Halison Correa Golias
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  2. Julio Cesar Polonio
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  3. Marcos Alessandro dos Santos Ribeiro
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Correspondence to João Alencar Pamphile.

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Golias, H.C., Polonio, J.C., dos Santos Ribeiro, M.A. et al. Tibouchina granulosa (Vell.) Cogn (Melastomataceae) as source of endophytic fungi: isolation, identification, and antiprotozoal activity of metabolites from Phyllosticta capitalensis. Braz J Microbiol 51, 557–569 (2020). https://doi.org/10.1007/s42770-019-00221-z

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