Abstract
Water scarcity poses a serious threat to agricultural cropping systems of arid and semi-arid regions, affecting many aspects of plant metabolism and performance. Bentonite is a natural and nontoxic soil amendment that can effectively ameliorate water utilization in crop production. The target of the present study was to monitor morphological, physiological, and biochemical responses of fenugreek (Trigonella foenum-graecum L.) to bentonite application under water deficit.
This study was arranged as factorial based on a completely randomized design with three replications. Treatments were three levels of bentonite application (0 g kg−1, 50 g kg−1, and 100 g kg−1 of soil) and different irrigation regimes (90%, 60%, and 30% field capacity (FC)). The traits were measured at the flowering stage.
Based on the results, compared to the corresponding control, in the presence of bentonite, drought-stressed fenugreek showed higher leaf area, length, and width and lower root length, volume, and diameter, dry and fresh weight. In addition, bentonite application enhanced relative water content (RWC) and photosynthetic pigments (chlorophyll a, b, and total) and minimized drought stress indexes such as electrolyte leakage (EL), osmolytes (soluble carbohydrates and proline), protein content, hydrogen peroxide (H2O2), malondialdehyde (MDA), antioxidant enzymes, catalase (CAT) and polyphenol oxidase (PPO), total phenol, and antioxidant activity under severe drought stress.
This study demonstrated the high efficacy of bentonite for increasing drought tolerance in fenugreek. However, the intensity of the positive response of plants to the use of bentonite is related to the severity of drought stress.
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Abbreviations
- FC:
-
Field capacity
- RWC:
-
Relative water content
- EL:
-
Electrolyte leakage
- H2O2 :
-
Hydrogen peroxide
- MDA:
-
Malondialdehyde
- CAT:
-
Catalase
- PPO:
-
Polyphenol oxidase
- ROS:
-
Reactive oxygen species
- SOD:
-
Superoxide dismutase
- SAP:
-
Super absorbent polymer
References
Abdulmajeed AM, Alnusairi GSH, Alharbi MH, Almushhin A, Hasan MdM, Soliman MH (2021) Alleviation of copper phytotoxicity by acetylsalicylic acid and nitric oxide application in mung bean involves the up-regulation of antioxidants, osmolytes and glyoxalase system. J Plant Interact 16:201–212. https://doi.org/10.1080/17429145.2021.1922771
Ahmed CB, Rouina BB, Sensoy S, Boukhris M, Abdallah FB (2009) Changes in gas exchange, proline accumulation and antioxidative enzyme activities in three olive cultivars under contrasting water availability regimes. Environ Exp Bot 67:345–352. https://doi.org/10.1016/j.envexpbot.2009.07.006
Babaei K, Moghaddam M, Farhadi N, Pirbalouti AG (2021) Morphological, physiological and phytochemical responses of Mexican marigold (Tagetes minuta L.) to drought stress. Sci Hortic 284:110116. https://doi.org/10.1016/j.scienta.2021.110116
Bandian L, Saeb H, Abedy B (2016) Effect of bentonite on growth indices and physiological traits of spinach (Spinacia oleracea L) under drought stress. J Product Develop 2:1–6
Bandian L, Nemati H, Moghaddam M (2019) Effects of bentonite application and urea fertilization time on growth, development and nitrate accumulation in Spinach (Spinacia oleraceae L.). Commun Soil Sci Plant Anal 50:1–9. https://doi.org/10.1080/00103624.2018.1538373
Başak H (2020) The effects of super absorbent polymer application on the physiological and biochemical properties of tomato (Solanum lycopersicum L.) plants grown by soilless agriculture technique. Appl Ecol Environ Res 18:5907–592. https://doi.org/10.15666/aeer/1804_59075921
Basu SK, Zandi P, Cetzal-Ix W (2019) Fenugreek (Trigonella foenum-graecum L.): Distribution, genetic diversity, and potential to serve as an industrial crop for the global pharmaceutical, nutraceutical, and functional food industries, the role of functional food security in global health. Elsevier, pp. 471–497. https://doi.org/10.1016/B978-0-12-813148-0.00028-1
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207. https://doi.org/10.1007/BF00018060
Beniwal RS, Hooda MS, Polle A (2011) Amelioration of planting stress by soil amendment with a hydrogel–mycorrhiza mixture for early establishment of beech (Fagus sylvatica L.) seedlings. Ann for Sci 68:803–810. https://doi.org/10.1007/s13595-011-0077-z
Besharati J, Shirmardi M, Meftahizadeh H, Ardakani MD, Ghorbanpour M (2021) Changes in growth and quality performance of Roselle (Hibiscus sabdariffa L.) in response to soil amendments with hydrogel and compost under drought stress. S Afr J Bot (in Press). https://doi.org/10.1016/j.sajb.2021.03.018
Bitarafan Z, Asghari HR, Hasanloo T, Gholami A, Moradi F, Khakimov B, Liu F, Andreasen C (2019) The effect of charcoal on medicinal compounds of seeds of fenugreek (Trigonella foenum-graecum L.) exposed to drought stress. Ind Crops Prod 131:323–329. https://doi.org/10.1016/j.indcrop.2019.02.003
Boeckx T, Webster R, Winters AL, Webb KJ, Gay A, Kingston-Smith AH (2015) Polyphenol oxidase-mediated protection against oxidative stress is not associated with enhanced photosynthetic efficiency. Ann Bot 116:529–540. https://doi.org/10.1093/aob/mcv081
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. https://doi.org/10.1006/abio.1976.9999
Chaeikar SS, Marzvan S, Khiavi SJ, Rahimi M (2020) Changes in growth, biochemical, and chemical characteristics and alteration of the antioxidant defense system in the leaves of tea clones (Camellia sinensis L.) under drought stress. Sci Hortic 265:109257. https://doi.org/10.1016/j.scienta.2020.109257
Chauhan J, Singhal RK, Kakralya BL, Kumar S, Sodani R (2017) Evaluation of yield and yield attributes of fenugreek (Trigonella foenum graecum) genotypes under drought conditions. Int J Pure App Biosci 5:477–484. https://doi.org/10.18782/2320-7051.4019
Cherki GH, Foursy A, Fares K (2002) Effects of salt stress on growth inorganic ions and proline accumulation in relation to osmotic adjustment in five sugar beet cultivars. Environ Exp Bot 47:39–50. https://doi.org/10.1016/S0098-8472(01)00109-5
Davey M, Stals E, Panis B, Keulemans J, Swennen R (2005) High-throughput determination of malondialdehyde in plant tissues. Anal Biochem 347:201–207. https://doi.org/10.1016/j.ab.2005.09.041
Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101. https://doi.org/10.1093/jxb/32.1.93
Dunn J, Hunt L, Afsharinafar M, Meselmani MA, Mitchell A, Howells R, Wallington E, Fleming AJ, Gray JE (2019) Reduced stomatal density in bread wheat leads to increased water-use efficiency. J Exp Bot 70:4737–4748. https://doi.org/10.1093/jxb/erz248
Farooq M, Wahid A, Kobayashi N, Fujita D, Basra S (2009) Plant drought stress: effects, mechanisms and management. Agron Sustain Dev 29:185–212. https://doi.org/10.1051/agro:2008021
Foyer CH, Noctor G (2005) Oxidant and antioxidant signaling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell Environ 28:1056–1071. https://doi.org/10.1111/j.1365-3040.2005.01327.x
Gapińska M, Skłodowska M, Gabara B (2008) Effect of short-and long-term salinity on the activities of antioxidative enzymes and lipid peroxidation in tomato roots. Acta Physiol Plant 30:11. https://doi.org/10.1007/s11738-007-0072-z
Hasan MM, Alharby HF, Uddin MN, Ali MA, Anwar Y, Fang XW, Hakeem KR, Alzahrani Y, Hajar AS (2020) Magnetized water confers drought stress tolerance in Moringa biotype via modulation of growth, gas exchange, lipid peroxidation and antioxidant activity. Pol J Environ Stud 29:1625–1636. https://doi.org/10.15244/pjoes/110347
Hasan MM, Ali MA, Soliman MH, Alqarawi AA, Abd Allah EF, Fang XW (2020b) Insights into 28-homobrassinolide (HBR)-mediated redox homeostasis, AsA–GSH cycle, and methylglyoxal detoxification in soybean under drought-induced oxidative stress. J Plant Interact 15:371–385. https://doi.org/10.1080/17429145.2020.1832267
Hasan MM, Skalicky M, Jahan MS, Hossain MN, Anwar Z, Nie ZF, Alabdallah NM, Brestic M, Hejnak V, Fang XW (2021) Spermine: its emerging role in regulating drought stress responses in plants. Cells 10:261. https://doi.org/10.3390/cells10020261
Hosseini MS, Samsampour D, Ebrahimi M, Abadía J, Khanahmadi M (2018) Effect of drought stress on growth parameters, osmolyte contents, antioxidant enzymes and glycyrrhizin synthesis in licorice (Glycyrrhiza glabra L.) grown in the field. Phytochemistry 156:124–134. https://doi.org/10.1016/j.phytochem.2018.08.018
Irankhah S, Chitarra W, Nerva L, Antoniou C, Lumini E, Volpe V, Ganjeali A, Cheniany M, Mashreghi M, Fotopoulos V (2020) Impact of an arbuscular mycorrhizal fungal inoculum and exogenous MeJA on fenugreek secondary metabolite production under water deficit. Environ Exp Bot 176:104096. https://doi.org/10.1016/j.envexpbot.2020.104096
Jahan MS, Shu S, Wang Y, Hasan MM, El-Yazied AA, Alabdallah NM, Hajjar D, Altaf MA, Sun J, Guo S (2021) Melatonin pretreatment confers heat tolerance and repression of heat-induced senescence in tomato through the modulation of ABA and GA-mediated pathways. Front Plant Sci 12:650955. https://doi.org/10.3389/fpls.2021.650955
Kar M, Mishra D (1976) Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiol 57:315–319. https://doi.org/10.1104/pp.57.2.315
Kenawy E-R, Saad-Allah K, Hosny A (2018) Mitigation of drought stress on three summer crop species using the superabsorbent composite Gelatin-gp (AA-co-AM)/RH. Commun Soil Sci Plant Anal 49:2828–2842. https://doi.org/10.1080/00103624.2018.1546871
Khan A, Anwar Y, Hasan M, Iqbal A, Ali M, Alharby HF, Hakeem KR, Hasanuzzaman M (2017) Attenuation of drought stress in Brassica seedlings with exogenous application of Ca2+ and H2O2. Plants 6:20. https://doi.org/10.3390/plants6020020
Kyani A, Niknam V (2015) Comparative responses of two Trigonella species to salinity and drought stresses in vitro. Prog Biol Sci 5:233–248. https://doi.org/10.22059/PBS.2015.56041
Leung DW (2018) Studies of catalase in plants under abiotic stress, antioxidants and antioxidant enzymes in higher plants. Springer, pp. 27–39. https://doi.org/10.1007/978-3-319-75088-0_2
Li J, Liu L, Zhou H, Li M (2018) Improved viability of areca (Areca catechu L.) seedlings under drought stress using a superabsorbent polymer. HortScience 53:1872–1876. https://doi.org/10.21273/HORTSCI13586-18
Li S, Li M, Zhang W, Li Y, Ai X, Liu B, Li Q (2019) Effects of CO2 enrichment on photosynthetic characteristics and reactive oxygen species metabolism in leaves of cucumber seedlings under salt stress. Acta Ecol Sin 39:1–9. https://doi.org/10.5846/stxb201712212296
Ḿbarki N, Aissaoui F, Chehab H, Dabbaghi O, del Giudice T, Boujnah D, Mechri B (2019) Cultivar dependent impact of soil amendment with water retaining polymer on olive (Olea europaea L.) under two water regimes. Agric Water Manag 216:70–75. https://doi.org/10.1016/j.agwat.2019.01.016
Mi J, Gregorich EG, Xu S, McLaughlin NB, Ma B, Liu J (2021) Changes in soil biochemical properties following application of bentonite as a soil amendment. Eur J Soil Biol 102:103251. https://doi.org/10.1021/jf9805799
Moon J-H, Terao J (1998) Antioxidant activity of caffeic acid and dihydrocaffeic acid in lard and human low-density lipoprotein. J Agric Food Chem 46:5062–5065. https://doi.org/10.1021/jf9805799
Okunlola GO, Olatunji OA, Akinwale RO, Tariq A, Adelusi AA (2017) Physiological response of the three most cultivated pepper species (Capsicum spp.) in Africa to drought stress imposed at three stages of growth and development. Sci Hortic 224:198–205. https://doi.org/10.1016/j.scienta.2017.06.020
Oraee A, Tehranifar A (2020) Evaluating the potential drought tolerance of pansy through its physiological and biochemical responses to drought and recovery periods. Sci Hortic 265:109225. https://doi.org/10.1016/j.scienta.2020.109225
Paquin R, Lechasseur P (1979) Observations sur une méthode de dosage de la proline libre dans les extraits de plantes. Can J Bot 57:1851–1854. https://doi.org/10.1016/j.scienta.2020.109225
Parkash V, Singh S (2020) A review on potential plant-based water stress indicators for vegetable crops. Sustainability 12:3945. https://doi.org/10.3390/su12103945
Pirzad A, Shakiba MR, Zehtab-Salmasi S, Mohammadi SA, Darvishzadeh R, Samadi A (2011) Effect of water stress on leaf relative water content, chlorophyll, proline and soluble carbohydrates in Matricaria chamomilla L. J Med Plant Res 5:2483–2488. https://doi.org/10.5897/JMPR.9000503
Porcel R, Ruiz-Lozano JM (2004) Arbuscular mycorrhizal influence on leaf water potential, solute accumulation, and oxidative stress in soybean plants subjected to drought stress. J Exp Bot 55:1743–1750. https://doi.org/10.1093/jxb/erh188
Rosales-Serna R, Kohashi-Shibata J, Acosta-Gallegos JA, Trejo-López C, Ortiz-Cereceres JN, Kelly JD (2004) Biomass distribution, maturity acceleration and yield in drought-stressed common bean cultivars. Field Crops Res 85:203–211. https://doi.org/10.1016/S0378-4290(03)00161-8
Sagisaka S (1976) The occurrence of peroxide in a perennial plant, Populus gelrica. Plant Physiol 57:308–309. https://doi.org/10.1104/pp.57.2.308
Saha A, Sekharan S, Manna U (2020) Superabsorbent hydrogel (SAH) as a soil amendment for drought management: a review. Soil Tillage Res 204:104736. https://doi.org/10.1016/j.still.2020.104736
Saxena S, Kakani R, Sharma L, Agarwal D, John S, Sharma Y (2017) Genetic variation in seed quality and fatty acid composition of fenugreek (Trigonella foenum-graecum L.) genotypes grown under limited moisture conditions. Acta Physiol Plant 39:1–10. https://doi.org/10.1007/s11738-017-2522-6
Sayyari M, Ghanbari F (2012) Effects of super absorbent polymer A200 on the growth, yield and some physiological responses in sweet pepper (Capsicum annuum L.) under various irrigation regimes. Int J Agric Food Res 1:1–11. https://doi.org/10.24102/ijafr.v1i1.123
Sharghi A, Naghdi Badi H, Bolandnazar S, Mehrafarin A, Sarikhani MR (2018) Morphophysiological and phytochemical responses of fenugreek to plant growth promoting rhizobacteria (PGPR) under different soil water levels. Folia Hortic 30:215–228. https://doi.org/10.2478/fhort-2018-0019
Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144–158
Soltys-Kalina D, Plich J, Strzelczyk-Żyta D, Śliwka J, Marczewski W (2016) The effect of drought stress on the leaf relative water content and tuber yield of a half-sib family of ‘Katahdin’-derived potato cultivars. Breed Sci 66:328–331. https://doi.org/10.1270/jsbbs.66.328
Su L-Q, Li J-g, Xue H, Wang X-F (2017) Super absorbent polymer seed coatings promote seed germination and seedling growth of Caragana korshinskii in drought. J Zhejiang Univ Sci B 18:696–706. https://doi.org/10.1631/jzus.B1600350
Şükran D, Güneş T, Sivaci R (1998) Spectrophotometric determination of chlorophyll-A, B and total carotenoid contents of some algae species using different solvents. Turk J Bot 22:13–18
Talbi S, Rojas JA, Sahrawy M, Rodríguez-Serrano M, Cárdenas KE, Debouba M, Sandalio LM (2020) Effect of drought on growth, photosynthesis and total antioxidant capacity of the saharan plant Oudeneya africana. Environ Exp Bot 176:104099. https://doi.org/10.1016/j.envexpbot.2020.104099
Teutonico RA, Palta JP, Osborn TC (1993) In vitro freezing tolerance in relation to winter survival of rapeseed cultivars. Crop Sci 33:103–107. https://doi.org/10.2135/cropsci1993.0011183X003300010019x
Valizadeh GBA, Hossein NS, Ali T, Hojat E (2014) Evaluation of chlorophyll fluorescence and biochemical traits of lettuce under drought stress and super absorbent or bentonite application. J Stress Physiol Biochem 1:301–315
Zhang W, Cao Z, Xie Z, Lang D, Zhou L, Chu Y, Zhao Q, Zhang X, Zhao Y (2017) Effect of water stress on roots biomass and secondary metabolites in the medicinal plant Stellaria dichotoma L. var. lanceolata Bge. Sci Hortic 224:280–285. https://doi.org/10.1016/j.scienta.2017.06.030
Zulqurnain Haider M, Hussain S, Muhammad Adnan Ramzani P, Iqbal M, Iqbal M, Shahzad T, Fatima M, Ali Khan S, Khan I, Shahid M, Ibrahim M, Tanzeem Ull Haq HS, Mahmood F (2019) Bentonite and biochar mitigate Pb toxicity in Pisum sativum by reducing plant oxidative stress and Pb translocation. Plants 8:571. https://doi.org/10.3390/plants8120571
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Mohammadifard, F., Tarakemeh, A., Moghaddam, M. et al. Bentonite Mitigates the Adverse Effects of Drought Stress in Fenugreek (Trigonella foenum-graecum L.). J Soil Sci Plant Nutr 22, 1098–1111 (2022). https://doi.org/10.1007/s42729-021-00718-3
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DOI: https://doi.org/10.1007/s42729-021-00718-3