Skip to main content

Advertisement

SpringerLink
Log in

High frequency in vitro callogenesis and plant regeneration of Glycyrrhiza glabra L.

  • Research Articles
  • Published:
Vegetos Aims and scope Submit manuscript

Abstract

Glycyrrhiza glabra, member of Leguminosae family, is economically valuable due to its medicinal properties. Today it is considered as an endangered plant, therefore establishing a rapid and efficient system for micropropagation of this plant is desirable for their protection. The present study standardized the micropropagation protocol for G. glabra involving callus induction and shoots regeneration with explants viz. leaf and stem. Our finding suggests that the use of only one sterilant is not efficient for the sterilization of licorice explant. HgCl2 (0.1%) for 5 min and ethanol (70%) for 5 min was found best when used along with prior washing and surface sterilization with tween-20 and bavistin. Callus induction was achieved on MS medium containing various concentration of BAP, 2,4-D and NAA. Maximum callus induction was observed on MS medium containing 2 mg/l BAP, 0.5 mg/l 2,4-D and 50 mg/l ascorbic acid with both leaf and stem explants. Production of callus using leaf as an explant was better than that of stem. The development of shoots from callus was initiated on MS medium containing BAP and IAA. The most efficient shoot formation was observed with 4 mg/l BAP, 0.2 mg/l IAA and 60 mg/l ascorbic acid. Best rooting was recorded with 3 mg/l IBA. Histological aspect of indirect organogenesis of G. glabra were evaluated. Successful multiplication of shoots from explants leads to the production of improved quality and great quantity of planting material.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Abdelwahd R, Hakam N, Labhilili M, Udupa S (2008) Use of an adsorbent and antioxidants to reduce the effects of leached phenolics in in vitro plantlet regeneration of faba bean. Afr J Biotechnol 7(8):997–1002

    CAS  Google Scholar 

  • Ahmad I, Hussain T, Ashraf I, Nafees M, Rafay M, Iqbal M (2013a) Lethal effects of secondary metabolites in plant tissue culture. Am Eurasian J Agric Environ Sci 13(4):539–447

    CAS  Google Scholar 

  • Ahmad N, Faisal M, Anis M (2013b) Role of PGR on in vitro shoot propagation in Cyamopsis tetragonoloba L. (Taub.): a drought tolerant grain legume. Rend Lincei 24(1):7–12

    Article  Google Scholar 

  • Arockiasamy S, Prakash S, Ignacimuthu S (2002) Direct organogenesis from mature leaf and petiole explants of Eryngium foetidum L. Biol Plant 45(1):129–132

    Article  Google Scholar 

  • Babaei N, Abdullah NAP, Saleh G, Abdullah TL (2013) Control of contamination and explant browning in Curculigo latifolia in vitro cultures. J Med Plant Res 7(8):448–454

    CAS  Google Scholar 

  • Bidwell SD, Pederick JW, Sommer-Knudsen J, Woodrow IE (2001) Micropropagation of the nickel hyperaccumulator, Hybanthus floribundus (Family Violaceae). Plant Cell Tissue Organ Cult 67(1):89–92

    Article  CAS  Google Scholar 

  • Blumenthal M, Goldberg A, Brinckmann J (2000) Herbal medicine: expanded commission E monographs. American Botanical Council, Newton, pp 233–236

  • Chawla HS (2002) Introduction to plant biotechnology, 2nd rev edn. Science Publishers Inc., Enfield, pp 36–56

    Google Scholar 

  • Chen WH, Davey MR, Power JB, Cocking EC (1988) Control and maintenance of plant regeneration in sugarcane callus cultures. J Exp Bot 39:251–261

    Article  CAS  Google Scholar 

  • CIMAP (1995) In vitro propagation of licorice through multiple shoot formation. Newsletter 21(4):4–5

    Google Scholar 

  • Crusheva R, Parvanov R (1978) Album of rare protected plants. Zemizdat Bulgaria, pp 27–28

  • Dhandapani M, Kim DH, Hong SB (2008) Efficient plant regeneration via somatic embryogenesis and organogenesis from the explants of Catharanthus roseus. In Vitro Cell Dev Biol Plant 44(1):18–25

    Article  CAS  Google Scholar 

  • Dodds JH, Roberts LW (1985) Experiments in plant tissue culture, 2nd edn. Cambridge Univ. Press, Cambridge, p 232

    Google Scholar 

  • Duke JA (1981) Handbook of legumes of world economic importance. Plenum Press, New York, pp 90–92

    Book  Google Scholar 

  • Jain S, Kharya MD, Nayak S, Barik R (2008) Effect of antioxidants on callus browning of Glycyrrhiza glabra. J Nat Remedies 8(1):44–47

    Google Scholar 

  • Jaiswal N, Verma Y, Misra P (2017) Micropropagation and in vitro elicitation of licorice (Glycyrrhiza spp.). In Vitro Cell Dev Biol Plant 53:145–166

    Article  CAS  Google Scholar 

  • Jan A, Bhat KM, Bhat SJA, Mir MA, Bhat MA, Imtiyaz AW, Rather JA (2013) Surface sterilization method for reducing microbial contamination of field grown strawberry explants intended for in vitro culture. Afr J Biotechnol 12:5749–5753

    Google Scholar 

  • Johnson M, Wesely EG, Kavitha MS, Uma V (2011) Antibacterial activity of leaves and inter-nodal callus extracts of Mentha arvensis L. Asian Pac J Trop Med 4(3):196–200

    Article  CAS  PubMed  Google Scholar 

  • Kakutani K, Ozaki K, Watanabe H, Tomoda K (1999) Somatic embryogenesis and plantlet regeneration on several species of licorice. Plant Biotechnol 16(3):239–241

    Article  CAS  Google Scholar 

  • Kane M (2003) Bacterial and fungal indexing of tissue cultures. http://plant-tc.cfans.umn.edu/listserv/1996/log9612/indexing.htm. Accessed 3 Jan 2016

  • Kikowska M, Thiem B, Sliwinska E, Rewers M, Kowalczyk M, Stochmal A, Oleszek W (2014) The effect of nutritional factors and plant growth regulators on micropropagation and production of phenolic acids and saponins from plantlets and adventitious root cultures of Eryngium maritimum L. J Plant Growth Regul 33(4):809–819

    Article  CAS  Google Scholar 

  • Ko WH, Su CC, Chen CL, Chao CP (2009) Control of lethal browning of tissue culture plantlets of Cavendish banana cv. Formosana with ascorbic acid. Plant Cell Tissue Organ Cult 96(2):137–141

    Article  Google Scholar 

  • Kojoma M, Ohyama K, Seki H, Hiraoka Y, Asazu SN, Sawa S, Sekizaki H, Yoshida S, Muranaka T (2010) In vitro proliferation and triterpenoid characteristics of licorice (Glycyrrhiza uralensis Fischer, Leguminosae) stolons. Plant Biotechnol 27(1):59–66

    Article  CAS  Google Scholar 

  • Kovalenko P, Kurchii B (1998) Using of abscisic acid in the plant tissue culture of licorice Glycyrrhiza glabra L. electroporated protoplasts. II. In: International symposium on plant biotechnology, Kyiv, Ukraine, p 65

  • Lalabadi MA, Jelodar NB, Bagheri N (2013) Effect of plant hormones on callus induction of explant types in endangered medicinal herb Tashnedari. Int J Biosci 3(12):39–43

    Article  CAS  Google Scholar 

  • Lin GZ, Zhao XM, Hong SK, Lian YJ (2011) Somatic embryogenesis and shoot organogenesis in the medicinal plant Pulsatilla koreana Nakai. Plant Cell Tissue Organ Cult 106:93–103

    Article  CAS  Google Scholar 

  • Liu B, Su S, Wu Y, Li Y, Shan X, Li S, Liu H, Dong H, Ding M, Han J, Yuan Y (2015) Histological and transcript analyses of intact somatic embryos in an elite maize (Zea mays L.) inbred line Y423. Plant Physiol Biochem 92:81–91

    Article  CAS  PubMed  Google Scholar 

  • Misaghi IJ, Donndelinger CR (1990) Endophytic bacteria in symptom- free cotton plants. Phytopathology 80:808–811

    Article  Google Scholar 

  • Mousa NA, Siagura P, Wiryowidagdo S, Wagih ME (2007) Establishment of regenerative callus and cell suspension system of licorice (Glycyrrhiza glabra) for the production of the sweetener glycyrrhizin in vitro. Sugar Tech 9(1):72–82

    Article  CAS  Google Scholar 

  • Murashige T, Skoog E (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Plant Physiol 15(3):473–497

    Article  CAS  Google Scholar 

  • Murata M, Nishimura M, Murai N, Haruta M, Homma S, Itoh Y (2001) A transgenic apple callus showing reduced polyphenol oxidase activity and lower browning potential. Biosci Biotechnol Biochem 65(2):383–388

    Article  CAS  PubMed  Google Scholar 

  • Murthy HN, Hahn EJ, Paek KY (2008) Adventitious roots and secondary metabolism. Chin J Biotechnol 24(5):711–716

    Article  CAS  Google Scholar 

  • Nassiri A, Hosseinzadeh H (2008) Review of pharmacological effects of Glycyrrhiza sp. and its biological compounds. Phytother Res 22:709–724

    Article  CAS  Google Scholar 

  • Naz R, Anis M, Aref IM (2015) Management of cytokinin-auxin interactions for in vitro shoot proliferation of Althaeae officinalis L.: a valuable medicinal plant. Rend Lincei 26(3):323–334

    Article  Google Scholar 

  • Ndakidemi CF, Ndakidemi PA (2013) The potential for developing an in vitro method for propagating Brachylaena huillensis (Silver oak). Am J Res Commun 1:224–241

    Google Scholar 

  • Nicholson RL, Hammerschmidt R (1992) Phenolic compounds and their role in disease resistance. Annu Rev Phytopathol 30:369–389

    Article  CAS  Google Scholar 

  • Olukoga A, Donaldson D (1998) Historical perspective on health. The history of licorice: the plant, its extracts, cultivation, commercialisation and entomology. J R Soc Health 118(5):300–304

    Article  CAS  Google Scholar 

  • Omamor IB, Asemota AO, Eke CR, Ezia EI (2007) Fungal contaminants of the oil palm tissue culture in Nigerian Institute for Oil Palm Research (NIFOR). Afr J Agric Res 2:534–537

    Google Scholar 

  • Poudyal BK, Du G, Zhang Y, Liu J, Shi Q (2008) Studies on browning problem and phenols content on shoots of Yali, Aikansui and Abbe Fetel pears for in vitro culture. Front Agric China 2(3):321–330

    Article  Google Scholar 

  • Purohit SD, Dave A, Kukda G (1994) Micropropagation of safed musli (Chlorophytum borivilianum), a rare Indian medicinal herb. Plant Cell Tissue Organ Cult 39(1):93–96

    Article  Google Scholar 

  • Rani G, Grover IS (1999) In vitro callus induction and regeneration studies in Withania somnifera. Plant Cell Tissue Organ Cult 57(1):23–27

    Article  Google Scholar 

  • Rattanpal HS, Gill MIS, Sangwan AK (2011) Micropropagation of strawberry through meristem culture. Acta Hortic 890:149–154

    Article  Google Scholar 

  • Sharma G, Nautiya AR (2009) Influence of explants type and plant growth regulators on in vitro multiple shoots regeneration of a Laurel from Himalaya. Nat Sci 7:1–7

    Google Scholar 

  • Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation in plant tissue cultures in vitro. Symp Soc Exp Biol 11:118–131

    CAS  PubMed  Google Scholar 

  • Slazak B, Sliwinska E, Saluga M, Ronikier M, Bujak J, Slomka A, Goransson U, Kuta E (2015) Micropropagation of Viola uliginosa (Violaceae) for endangered species conservation and for somaclonal variation-enhanced cyclotide biosynthesis. Plant Cell Tissue Organ Cult 120(1):179–190

    Article  CAS  Google Scholar 

  • Thiem B, Kikowska M, Krawczyk A, Wieckowska B, Sliwinska E (2013) Phenolic acid and DNA contents of micropropagated Eryngium planum L. Plant Cell Tissue Organ Cult 114(2):197–206

    Article  CAS  Google Scholar 

  • Thomas TD (2008) The role of activated charcoal in plant tissue culture. Biotechnol Adv 26:618–631

    Article  CAS  PubMed  Google Scholar 

  • Tiwari S, Arya A, Kumar S (2012) Standardizing sterilization protocol and establishment of callus culture of sugarcane for enhanced plant regeneration in vitro. Res J Bot 7:1–7

    Article  CAS  Google Scholar 

  • Verma P, Mathur AK (2011) Direct shoot bud organogenesis and plant regeneration from pre-plasmolysed leaf explants in Catharanthus roseus. Plant Cell Tissue Organ Cult 106(3):401–408

    Article  CAS  Google Scholar 

  • Wani M, Pande S, More N (2010) Callus induction studies in Tridax procumbens L. Int J Biotechnol Appl 2(1):11–14

    Article  Google Scholar 

  • Wojcik A, Podstolki A (2007) Leaf explants response in in vitro culture of St. John’s wort (Hypericum perforatum L.). Acta Physiol Plant 29(2):151–156

    Article  CAS  Google Scholar 

  • Wongwicha W, Tanaka H, Shoyama Y, Tuvshintogtokh I, Putalun W (2008) Production of glycyrrhizin in callus cultures of Licorice. Z Naturforsch 63(5–6):413–417

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was supported and financed by Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad, Uttar Pradesh, India.

Author information

Authors and Affiliations

  1. Department of Biochemistry and Biochemical Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, Uttar Pradesh, 211007, India

    Nancy Jaiswal & Yashodhara Verma

  2. Department of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, Uttar Pradesh, 211007, India

    Pragati Misra

Authors
  1. Nancy Jaiswal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yashodhara Verma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pragati Misra
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nancy Jaiswal.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jaiswal, N., Verma, Y. & Misra, P. High frequency in vitro callogenesis and plant regeneration of Glycyrrhiza glabra L.. Vegetos 34, 495–504 (2021). https://doi.org/10.1007/s42535-021-00219-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42535-021-00219-9

Keywords

Search

Navigation