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The impact of biological soil disinfestation in conjunction with antagonists and organic materials on potato brown rot control

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Abstract

Biological Soil Disinfestation (BSD) is used to control soil-borne phytopathogens like Ralstonia solanacearum by introducing fresh organic matter and covering the soil with thick, opaque plastic sheets to induce soil anaerobiosis. In this study, BSD was implemented for the first time in Egypt to manage R. solanacearum in five naturally-infested fields. To improve BSD’s efficiency, biocontrol agents, including Pseudomonas putida (PD3142), P. fluorescens (PD3339), Acinetobacter baumannii (PD3138), and Stenotrophomonas maltophilia (PD4560), or plant-animal compost were applied after removing the plastic. The pathogen was eliminated when the soil oxygen concentration was less than or equal to 1%. However, this method resulted in a decrease in potato production. Application of P. putida and P. fluorescens after BSD led to a significant increase in potato production. Furthermore, BSD led to a significant decrease in fungal biodiversity, measured by the Shannon index (H’). PCR-DGGE analysis showed that P. putida and S. maltophilia reduced bacterial biodiversity as indicated by species richness (S) and H’. Next generation sequencing assays showed Moraxellaceae and Pseudomonadaceae decrease in their abundance after BSD treatment, while Bacillaceae were less affected . Moreover, applying of compost following BSD was associated with an increase in beneficial bacterial genera such as Luteolibacter, Dyadobacter, Sphingobium, Flavobacterium, and Pseudomonas.

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Data Availability

The data and material used during the current study are available from the author on reasonable request.

Change history

Abbreviations

BSD:

Biological soil disinfestation

Poor BSD:

Soil Oxygen concentration > 1

PD:

Plantenziektenkundige Dienst (Plant protection Service, Wageningen, the NL)

S :

Species richness

H’:

Shannon index

DGGE:

Denaturing gradient gel electrophoresis

NGS:

Next generation sequencing

CMC:

Carboxymethyl cellulose

ARC:

Agricultural Research Center

FITC:

Fluorescein Isothiocyanate

OM:

Organic matter

SFOM:

Soil mixed with the fresh OM

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Acknowledgements

This study was conducted as part of the “Rehabilitation of Nile Valley and Delta to produce brown rot-free potato suitable for exportation” project, funded by The Science, Technology & Innovation Funding Authority (STIFA27859) of the Egyptian Ministry of Scientific Research. The author is deeply appreciative of the financial support and collaborative efforts.

Professor Nabil S. Farag, Emeritus Professor at the Plant Pathology Research Institute (PPATHRI), ARC, Egypt, is acknowledged for his invaluable review of the work and insightful feedback. Special thanks are extended to Professor Ahmed A. Gomah, Emeritus Professor at PPATHRI, ARC, for his valuable contributions to field trials and his expert advice on experiments in the field.

Funding

The study was carried out as part of the “Rehabilitation of Nile Valley and Delta to produce brown rot-free potato suitable for exportation” project, which was funded by The Science, Technology & Innovation Funding Authority (STIFA27859) of the Egyptian Ministry of Scientific Research.

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  1. Bacterial Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center (ARC), Giza, Egypt

    Nevein A. S. Messiha

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Acknowledgements

This study was conducted as part of the “Rehabilitation of Nile Valley and Delta to produce brown rot-free potato suitable for exportation” project, funded by The Science, Technology & Innovation Funding Authority (STIFA27859) of the Egyptian Ministry of Scientific Research. The author is deeply appreciative of the financial support and collaborative efforts.

Professor Nabil S. Farag, Emeritus Professor at the Plant Pathology Research Institute (PPATHRI), ARC, Egypt, is acknowledged for his invaluable review of the work and insightful feedback. Special thanks are extended to Professor Ahmed A. Gomah, Emeritus Professor at PPATHRI, ARC, for his valuable contributions to field trials and his expert advice on experiments in the field.

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Messiha, N.A.S. The impact of biological soil disinfestation in conjunction with antagonists and organic materials on potato brown rot control. Indian Phytopathology 76, 1001–1014 (2023). https://doi.org/10.1007/s42360-023-00668-z

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