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Determining the antifungal activity and characterization of Bacillus siamensis AMU03 against Macrophomina phaseolina (Tassi) Goid

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Abstract

Plant pathogens caused severe economic losses to the agricultural farming. Members of the genus Bacillus are considered to be a natural laboratory for the production of biologically active molecules that are strong inhibitors for the growth of phytopathogens. GJ-01 bacterial strain was isolated from crude oil-contaminated soil and later identify as Bacillus siamensis AMU03 through morphological, biochemical tests and molecular sequencing method. Culture conditions provided for the biosurfactant activity was highest with surface activity had a pH of 7 at 37 °C during the 7-day incubation period. A significant level of > 50% pathogen growth was inhibited under dual culture and at 3% culture filtrate in food poisoning method. The chemical composition of the biosurfactant was elucidated by the use of Thin layer chromatography and Fourier transform–infrared spectroscopy, and it was adjudged that the extract belongs to lipopeptides-surfactin class. This may help in future research studies in the area of diseases management strategies.

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References

  • Anandaraj B, Thivakaran P (2010) Isolation and production of biosurfactant producing organism from oil spilled soil. J Biosci Technol 1(3):120 126

    Google Scholar 

  • Anis M, Abbasi MW, Zaki MJ (2010) Bioefficacy of microbial antagonists against Macrophomina phaseolina on a sunflower. Pak J Bot 42:2935–2940

    Google Scholar 

  • Asaka O, Shoda M (1996) Biocontrol of Rhizoctonia solani damping off of tomato with Bacillus subtilis RB14. Appl Environ Microbiol 62:4081–4085

    CAS  PubMed  PubMed Central  Google Scholar 

  • Banat IM, Franzetti A, Gandolfi I, Bestetti G, Martinotti MG, Fracchia L, Smith TJ, Marchant R (2010) Microbial biosurfactants production, applications and future potential. Appl Microbiol Biotechnol 87:427–444

    CAS  PubMed  Google Scholar 

  • Bayoumi RA, Haroun BM, Ghazal EA, Mahe YA (2010) Structural analysis and characterization of biosurfactants produced by some crude oil utilizing bacterial strains. Aust J Basic Appl Sci 4:3484–3498

    CAS  Google Scholar 

  • Bezza FA, Chirwa EMN (2015) Production and applications of lipopeptide biosurfactant for bioremediation and oil recovery by Bacillus subtilis CN2. Biochem Eng J 101:168–78

    CAS  Google Scholar 

  • Hussain T, Khan AA (2018a) Bacillus firmus HussainT: Lab. 66: A new Biosurfactant producing bacteria for the biocontrol of late blight of potato caused by Phytophthora infestans (Mont.) de Bary. In: National Seminar on new paradigms of plant health management: sustainable food security under climatic scenario from 17th Nov to 19th Nov 2018 at Bihar Agricultural University, Sabour (Bhagalpur), Bihar, India under Indian Phytopathological Society, Eastern Zone region Annual meeting. OP, p 60

  • Bodour AA, Maier RM (1998) Application of a modified dropcollapse technique for surfactant quantification and screening of biosurfactant- producing microorganisms. J Microbiol Methods 32:273–280

    CAS  Google Scholar 

  • Chandankere R, Yao J, Cai M, Masakorala K, Jain AK, Choi MMF (2014) Properties and characterization of biosurfactant in crude oil biodegradation by bacterium Bacillus methylotrophicus UST. Fuel 122:140–148

    CAS  Google Scholar 

  • Ferradji FZ, Mnif S, Badis A, Rebbani S, Fodil D, Eddouaouda K (2014) Naphthalene and crude oil degradation by biosurfactant producing Streptomyces spp. isolated from Mitidja plain soil (North of Algeria). Int Biodeterior Biodegrad 86:300–308

    CAS  Google Scholar 

  • Ghojavand H, Vahabzadeh F, Mehranian M, Radmehr M, Shahraki KA, Zolfagharian F, Emadi MA, Roayaei E (2008) Isolation of thermotolerant, halotolerant, facultative biosurfactant-producing bacteria. Appl Microbiol Biotechnol 80:1073–1085

    CAS  PubMed  Google Scholar 

  • Gravel V, Martinez C, Antoun H, Tweddel RJ (2005) Antagonistic microorganisms with the ability to control Pythium damping-off of tomato seeds in rockwool. Biocontrol 50:771–786

    Google Scholar 

  • Grover RK, Moore JD (1962) Toximetric studies of fungicides of brown rot organism Sclerotinia fruticola and S. laxa. Phytopathology 52:876–880

    CAS  Google Scholar 

  • Heimpel GE, Mills N (2017) Biological control-ecology and applications. Cambridge University Press, Cambridge

    Google Scholar 

  • Hultberg M, Bengtsson T, Liljeroth E (2010) Late blight on potato is suppressed by the biosurfactant-producing strain Pseudomonas koreensis 2.74 and its biosurfactant. BioControl 55:543–550

    CAS  Google Scholar 

  • Hussain T, Khan AA (2018b) A combination of rapid and easy assays of biosurfactant producing bacteria strain isolated from automobiles repairing workshop in Aligarh. Proc Voronezh State Univ Eng Technol 80(3):153–163

    Google Scholar 

  • Hussain T, Khan AA (2020) Bacillus subtilis HussainT-AMU and its antifungal activity against Potato black scurf caused by Rhizoctonia solani. Biocatal Agric Biotechnol 23:101433

    Google Scholar 

  • Ibrahim ML, Ijah UJJ, Manga SB, Bilbis LS, Umar S (2013) Production and partial characterization of biosurfactant produced by crude oil degrading bacteria. Int Biodeterior Biodegrad 81:28–34

    CAS  Google Scholar 

  • Jana TK, Sharma TR, Singh NK (2005) SSR-based detection of genetic variability in the charcoal kinase of Trichoderma virens, is involved in biocontrol properties and repression of conidiation, pathogenicity, pycnidium production and chlorate utilization for root rot pathogen Macrophomina phaseolina. Can J Bot 73:1596–1603

    Google Scholar 

  • Jharia HK, Duhoon SS (2000) Interactions between antagonists and Macrophomina phaseolina causing stem and root rot in sesame. In: Paper presented in National Seminar on oilseeds and oils research and development need in the Millennium Held at DOR, Hydrabad: 218(Ab)

  • Jones TS (1949) Chemical evidence for the multiplicity of the antibiotics produced by Bacillus polymyxa. Ann N Y Acad Sci 51:909–916

    CAS  PubMed  Google Scholar 

  • Juwarkar AA, KV Dubey A, Nair, Singh SK (2008) Bioremediation of multi-metal contaminated soil using biosurfactant—a novel approach. Ind J Microbiol 48:142–146

    CAS  Google Scholar 

  • Kaisar SAKM, Das SN (1988) Physical factors that influence the growth and spread of charcoal rot pathogen (Macrophomina phaseolina) infecting maize. J Phytopathol 123:47–51

    Google Scholar 

  • Khan SN (2007) Macrophomina phaseolina as causal agent for charcoal rot of sunflower. Mycopath 5(2):111–118

    Google Scholar 

  • Khirood D, Paramjit KJ (2012) In vitro efficacy of Trichoderma viride against Sclerotium rolfsii and Macrophomina phaseolina. Sci Biol 4(4):39–44

    Google Scholar 

  • Krishnayya PV, Grewal PS (2002) Effect of neem and selected fungicides on viability and virulence of the entomopathogenic nematode steinernema feltiae. Biocontrol Sci Technol 12:259–266

    Google Scholar 

  • Yoon et al (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 67:1613–1617

    CAS  PubMed  PubMed Central  Google Scholar 

  • Lee D, Kim IY, Saha S, Choi KS (2016) Paraptosis in the anti-cancer arsenal of natural products. Pharmacol Ther 162:120–133

    CAS  PubMed  Google Scholar 

  • Lokesha NM, Benagi VI (2007) Biological management of pigeonpea dry root rot caused by Macrophomina phaseolina. Karnataka J Agric Sci 20:54–56

    Google Scholar 

  • Morikawa M, Hirata Y, Imanaka T (2000) A study on the structure function relationship of lipopeptide biosurfactants. Biochim Biophys Acta 1488:211–218

    CAS  PubMed  Google Scholar 

  • Nakhate PH, Yadav VK, Pathak AN (2013) A review on daptomycin: the first US-FDA approved lipopeptide anti-biotics. J Sci Innov Res 2:970–980

    Google Scholar 

  • Padmapriya B, Rajeswari T, Suganthi S, Rajeswari T, Jayalakshmi S (2011) Biosurfactant production and plasmid isolation from newly isolated hydrocarbonoclastic bacteria Proteus inconstans. Int J Pharm Biol Arch 2:784–790

    Google Scholar 

  • Pal KK, Tilak KVBR, Saxena AK, Dey R, Singh CS (2001) Suppression of maize root diseases caused by M. phaseolina, Fusarium moniliforme and Fusarium graminearum by plant growth promoting rhizo bacteria. Microbiol Res 156:209223

    Google Scholar 

  • Raaijmakers JM, Mazzola M (2012) Diversity and natural functions of antibiotics produced by beneficial and plant pathogenic bacteria. Annu Rev Phytopathol 50:403–424

    CAS  PubMed  Google Scholar 

  • Rabindran R, Vidhyasekaran P (1996) Development of a formulation of Pseudomonas fluorescens PfALR2 for management of rice sheath blight. Crop Protect 15:715–721

    Google Scholar 

  • Satpute SK, Bhawsar BD, Dhakephalkar PK, Chopade BA (2008) Assessment of different screening methods for selecting biosurfactant producing marine bacteria. Indian J Mar Sci 37:243–250

    CAS  Google Scholar 

  • Silva CL. Ciampi yL, Burzio (1997) Caracterización preliminar de un metabolito de Bacillus sp. con actividad antibiótica. In VI Congreso Nacional de Fitopatología. Talca Resúmenes Simiente 67:68–95

    Google Scholar 

  • Singh G, Verma RK (2015) Compatibility of bioagents and neem products against root rot of soybean. J Mycopathol Res 43(2):211–214

    Google Scholar 

  • Singh N, Pandey P, Dubey RC, Maheshwari DK (2008) Biological control of root rot fungus Macrophomina phaseolina and growth enhancement of Pinus roxburghii (Sarg.) by rhizosphere competent Bacillus subtilis BN1. World J Microbiol Biotechnol 24(9):1669–1679

    Google Scholar 

  • Snook ME, Mitchell T, Hinton DM, CW, Bacon (2009) Isolation and characterization of Leu7-surfactin from the endophytic bacterium Bacillus mojavensis RRC 101, a biocontrol agent for Fusarium verticillioides. J Agric Food Chem 57:4287–4292

    CAS  PubMed  Google Scholar 

  • Sriram MI, Kalishwaralal K, Deepak V, Gracerosepat R, Srisakthi K, Gurunathan S (2011) Biofilm inhibition and antimicrobial action of lipopeptide biosurfactant produced by heavy metal tolerant strain Bacillus cereus NK1. Colloid Surf B Interfaces 85:174–181

    CAS  Google Scholar 

  • Srivastava AK, Singh T, Jana TK, Arora DK (2001) Induced resistance and control of charcoal rot in chickpea. Plant Dis 84:377–393

    Google Scholar 

  • Sundravadana S (2002) Management of blackgram (Vigna mungo (L). Hepper) root rot (Macrophomina phaseolina (Tassi.) Goid with bioagents and nutrients. M.Sc. Thesis. Department of Plant Pathology, Tamil Nadu Agricultural University, Tamil Nadu

  • Szczech M, Shoda M (2006) The effect of mode of application of Bacillus subtilis RB14-C on its efficacy as a biocontrol agent against Rhizoctonia solani. J Phytopathol 154:370–377

    CAS  Google Scholar 

  • Vidyasekaran P, Muthamilan M (1995) Development of formulations of Pseudomonas fluorescens for control of chickpea wilt. Plant Dis 79:782–786

    Google Scholar 

  • Vincent JM (1947) Distortion of fungal hyphae in the presence of certain inhibitors. Nature 159:850

    CAS  PubMed  Google Scholar 

  • Vyas SC, Patel MC (2015) Integrated biological and chemical control of dry root rot of chickpea. Indian J Mycol Plant Pathol 24(2):132–134

    Google Scholar 

  • Youssef NH, Duncan KE, Nagle DP, Savage KN, Knapp RM, McInerney MJJ (2004) Comparison of methods to detect biosurfactant production by diverse microorganisms. Microbiol Methods 56(3):339–347

    CAS  Google Scholar 

  • Zape AS, Gade RM, Singh R, Deshmukh VA (2014) Efficacy of different antagonist against the Sclerotium rolfsii. Rhizoctonia solani and Fusarium solani. Bioscan 9(4):1431–1434

    Google Scholar 

  • Zhang J, Xue Q, Gao H, Lia H, Wang P (2016) Production of lipopeptide biosurfactants by Bacillus atrophaeus 5-2a and their potential use in microbial enhanced oil recovery. Microb Cell Fact 15:168

    PubMed  PubMed Central  Google Scholar 

  • Zhao HB, Shao DY, Jiang CM, Shi JL, Li Q, Huang QS, Rajoka MSR, Yang H, Jin ML (2017) Biological activity of lipopeptides from Bacillus. Appl Microbiol Biotechnol 101(15):5951–5960

    CAS  PubMed  Google Scholar 

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Acknowledgements

The first author is very thankful to Startup Grant, by Dept. of Science and Technology, Indian Government, New Delhi for providing the financial grants received under File No. DST-SERB-NPDF/2016/001409 and Dept. of Botany, Aligarh Muslim University, Aligarh, U.P for proving all the support, infrastructure, Dept. of Chemistry, AMU, Aligarh for FT-IR facility.

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  1. Plant Pathology and Nematology Section, Department of Botany, Aligarh Muslim University, Aligarh, UP, 202 002, India

    Touseef Hussain & Abrar Ahmad Khan

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  1. Touseef Hussain
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Correspondence to Touseef Hussain.

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Hussain, T., Khan, A.A. Determining the antifungal activity and characterization of Bacillus siamensis AMU03 against Macrophomina phaseolina (Tassi) Goid. Indian Phytopathology 73, 507–516 (2020). https://doi.org/10.1007/s42360-020-00239-6

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