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Multifarious allelochemicals exhibiting antifungal activity from Bacillus subtilis MBCU5

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Abstract

A potential antagonist, designated strain Bacillus subtilis MBCU5 was previously isolated from vermicompost-amended soils of Gandhinagar, Gujarat, India. Crude allelochemicals from strain MBCU5 displayed strong antifungal activity against Macrophomina phaseolina as well as Rhizoctonia solani. These crude allelochemicals were tentatively identified as iturin, fengycin and surfactin through TLC and HPTLC analysis. Lipopeptides produced by MBCU5 were identified by MALDI-TOF–MS and LC–ESI–MS/MS analysis showed that iturin homologues (m/z 1020–1120), surfactin (m/z 1008.7 and m/z 1022.7), fengycin A and fengycin B (m/z 1400–1550) types of allelochemicals which are responsible for antifungal activity against pathogens. PCR analysis showed presence of genes (i.e. Iturin A synthetase KJ531680 and Surfactin synthetase KJ601726) involved in the biosynthesis of allelochemicals. Many reports showed lipopeptides from Bacillus species; this is the first report executed of multifarious allelochemicals from vermicompost-amended soil due to the presence of predominant Bacillus species.

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Acknowledgements

The financial support from Department of Science and Technology (DST), New Delhi, India under Women Scientist Scheme (WOS-A) (Grant No. SR/WOS-A/LS-186/2010) is gratefully acknowledged. The authors also thank to Dr. Khyati Pathak and Prof. H. B. Singh for their valuable guidance and expertise.

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Authors and Affiliations

  1. Department of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad, 380 009, India

    Urja Pandya & Meenu Saraf

  2. Molecular Biophysics Unit, Indian Institute of Science, Bangalore, 560012, India

    Sunita Prakash

  3. Department of Biotechnology and Bioinformatics Center, Barkatullah University, Bhopal, Madhya Pradesh, 462026, India

    Kishor Shende

  4. Department of Biotechnology, School of Life Science, North Maharashtra University, Jalgaon, 425001, India

    Umesh Dhuldhaj

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  1. Urja Pandya
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  2. Sunita Prakash
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  3. Kishor Shende
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  4. Umesh Dhuldhaj
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  5. Meenu Saraf
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Correspondence to Meenu Saraf.

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Pandya, U., Prakash, S., Shende, K. et al. Multifarious allelochemicals exhibiting antifungal activity from Bacillus subtilis MBCU5. 3 Biotech 7, 175 (2017). https://doi.org/10.1007/s13205-017-0827-1

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