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Morphological characterization and screening for sheath blight resistance using Indian isolates of Rhizoctonia solani AG1IA

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Abstract

Sheath blight of rice caused by Rhizoctonia solani AG1IA is one of the devastating fungal diseases. One hundred and twelve R. solani AG1IA isolates collected from Uttar Pradesh, Uttarakhand and Haryana states of India were studied for their morphological characterization. All the isolates of R. solani AG1IA were classified into five groups on the basis of sclerotial arrangements i.e. Group-1, Group-2, Group-3, Group-4 and Group-5 which contains 28, 35, 44, 3 and 2 isolates, respectively. Majority of the isolates were spatial in growth pattern (38.3%), fast growing (40.1%), light brown colony colour (72.3%), scattered sclerotia (39.2%), deep dark brown sclerotia (91.0%), sclerotial clump formation (32.1%) and micro sized sclerotia (56.2%). The number of sclerotia produced was in the range of 3.7–10.7/5 mm mycelium disc. Two isolates RSV10 and RSJ79 did not produce any sclerotia. Time of initiation of sclerotia formation was varied between 4 and 6 days. Sixty-five isolates showed sclerotia formation on under surface of lid. Principal component analysis (PCA) of growth rate and number of sclerotia of R. solani AG1IA showed 100.00 and 86.84% variation, respectively. A field trial with 261 rice germplasm was conducted during the 2013–2014 and 2014–2015 Kharif seasons to screen the R. solani AG1IA resistant germplasm. Mean percent disease index (PDI) varied between 22.95 and 27.40%. On the basis of AUDPC (area under disease progress curve) values, rice germplasm lines belonged to 5 groups i.e. resistant, 57 (262.93–957.92), moderately resistant, 169 (957.93–1220.87), moderately susceptible, 14 (1220.88–1490.81), susceptible, 18 (1490.82–1753.75) and highly susceptible, 3 (1753.76–2016.69).

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Acknowledgements

The authors are thankful to the Head, Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India for supporting this work.

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Authors and Affiliations

  1. ICAR-National Bureau of Agriculturally Important Microorganisms, Kushmaur, Mau Nath Bhanjan, Mau, Uttar Pradesh, 275 101, India

    Sanjay K. Goswami

  2. Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, 221 005, India

    Vineeta Singh

  3. ICAR-Indian Institute of Wheat and Barley Research, Karnal, Haryana, 132 001, India

    P. L. Kashyap

  4. Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, 221 005, India

    P. K. Singh

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  1. Sanjay K. Goswami
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Correspondence to Sanjay K. Goswami.

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Goswami, S.K., Singh, V., Kashyap, P.L. et al. Morphological characterization and screening for sheath blight resistance using Indian isolates of Rhizoctonia solani AG1IA. Indian Phytopathology 72, 107–124 (2019). https://doi.org/10.1007/s42360-018-0103-2

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